杉虎杂交斑5S rRNA基因甲基化分析  

Methylation Analysis of 5S rRNA Gene in the Hybrid Grouper(Epinephelus fuscoguttatus×Epinephelus polyphekadion)

作　　者：曹柳 黄潇庆 陈菁苗 卢艳 黄海 CAO Liu;HUANG Xiaoqing;CHEN Jingmiao;LU Yan;HUANG Hai(Yazhou Bay Innovation Institute,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education,Hainan Tropical Ocean University,Sanya Hainan 572022,China;College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya Hainan 572022,China)

机构地区：[1]海南热带海洋学院崖州湾创新研究院,海南三亚572022 [2]海南热带海洋学院海南省热带海洋渔业资源保护与利用重点实验室,海南三亚572022 [3]海南热带海洋学院热带海洋生物资源利用与保护教育部重点实验室,海南三亚572022 [4]海南热带海洋学院水产与生命学院,海南三亚572022

出　　处：《海南热带海洋学院学报》2024年第2期11-17,共7页Journal of Hainan Tropical Ocean University

基　　金：海南热带海洋学院崖州湾创新研究院青年项目(2022CXYQNXM06);海南热带海洋学院引进人才科研启动项目(RHDRC202010);海南省重大科技计划项目(ZDKJ2021017);海南省自然科学基金青年基金项目(321QN263)。

摘　　要：核糖体RNA是构成核糖体的重要组成部分,在蛋白质合成过程中起着重要作用。利用雌性棕点石斑鱼(Epinephelus fuscoguttatus)与雄性清水石斑鱼(Epinephelus polyphekadion)杂交,获得在生长、肉质等方面具有一定优势的子代——杉虎杂交斑。对杉虎杂交斑及其亲本5S rRNA基因编码区前1 000 bp、编码区以及非转录区的DNA甲基化水平进行测定和比较分析。结果表明,杉虎杂交斑5S rRNA基因整体甲基化水平(0.418)高于棕点石斑鱼(0.379)和清水石斑鱼(0.037),但与母本棕点石斑鱼不具有显著性差异,与父本清水石斑鱼存在显著性差异。对5S rRNA基因编码区和非转录区的甲基化位点进行分析发现,在棕点石斑鱼与杉虎杂交斑中所有甲基化位点是一致的,但在清水石斑鱼中均未发现这些位点,表明杉虎杂交斑的5S rRNA基因甲基化模式与母本棕点石斑鱼一致。结果阐明了杉虎杂交斑5S rRNA基因的DNA甲基化水平和模式,为石斑鱼杂交育种提供理论基础。Ribosomal RNA(rRNA)is an important component of ribosomal which plays an important role in protein synthesis.The hybrid grouper derived from the hybridization of Epinephelus fuscoguttatus×Epinephelus polyphekadion,exhib⁃its a certain of advantages in growth and meat quality.The DNA methylation levels of the first 1000 bp of the coding re⁃gion of 5S rRNA gene,coding region and non-transcribed spacer of 5S rRNA gene were measured and analyzed contras⁃tively between the hybrid grouper and its parents.Results showed that the overall methylation level of 5S rRNA gene in the hybrid grouper(0.418)was higher than that of E.fuscoguttatus(0.379)and E.polyphekadion(0.037).While there was no significant difference between the hybrid grouper and E.fuscoguttatus,significant difference was detected between the hybrid grouper and E.polyphekadion.Comparative analysis of DNA methylation level at each CpG site in the coding region and non-transcribed spacer revealed that all methylation sites co⁃existed in both the hybrid grouper and E.fuscoguttatus,but were not found in E.polyphekadion.These results indicate that the methylation pattern of the 5S rRNA gene in the hy⁃brid grouper was similar to that of E.fuscoguttatus.The observations elucidate the DNA methylation level and pattern of 5S rRNA gene in the hybrid grouper,and provide a theoretical basis for cross⁃breeding in grouper.

关 键 词：石斑鱼 杂交 5S rRNA基因 DNA甲基化 

分 类 号：S917.4[农业科学—水产科学]