台湾泥鳅腐皮病霍乱弧菌的分离与鉴定  

Isolation and Identification of V.cholerae from the Rotting Skin Disease of Paramisgurnus dabryanus ssp.

作　　者：杨宁 姜芳燕 陈攀 马军 陈燕 曹柳 侯兴蓉 YANG Ning;JIANG Fangyan;CHEN Pan;MA Jun;CHEN Yan;CAO Liu;HOU Xingrong(Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Yazhou Bay Innovation Institute,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Tropical Biodiversity and Bioresource Utilization Laboratory,Qiongtai Normal University,Haikou 571132,China)

机构地区：[1]海南热带海洋学院热带海洋生物资源利用与保护教育部重点实验室,海南三亚572022 [2]海南热带海洋学院海南省热带海洋渔业资源保护与利用重点实验室,海南三亚572022 [3]海南热带海洋学院崖州湾创新研究院,海南三亚572022 [4]琼台师范学院热带生物多样性与资源利用实验室,海口571127

出　　处：《海南热带海洋学院学报》2024年第2期18-24,共7页Journal of Hainan Tropical Ocean University

基　　金：三亚市院地科技合作项目(2016YD20);海南热带海洋学院科研启动项目(RHDRC202101);海南省院士创新平台资金资助项目。

摘　　要：为研究台湾泥鳅(Paramisgumus dabryanus ssp.)腐皮病的病原,对其腐皮等病变部位进行细菌分离纯化,获得1株优势菌株,命名为P1,利用革兰氏染色观察其形态特征,利用PCR扩增技术、16S rRNA基因测序,得到病原菌的序列,用MEGA软件构建系统进化树,再结合生理生化分析结果鉴定菌种名称。结果发现:革兰氏染色阴性,弧形,两端钝圆;菌体长1~1.5μm,宽度1~1.2μm,有菌丝和鞭毛,16S rRNA基因1 395 bp。建立系统进化树后发现,P1与霍乱弧菌处于同一分支;生理生化实验结果与霍乱弧菌一致,从而鉴定该菌株为霍乱弧菌(Vibrio cholerae)。药敏实验结果表明,该分离菌株对头孢哌酮、卡那霉菌、万古霉素等10种抗生素高度敏感;对四环素中度敏感;对呋喃唑酮、复方新诺明、克林霉素等17种抗生素耐药。实验结果为台湾泥鳅腐皮病的病原鉴定与药物防治提供参考。In order to study the pathogen of P.dabryanus ssp.,a dominant strain named P1 was obtained by bacterial isolation and purification of its rotting skin and other lesions.The morphological characteristics of the strain were observed by Gram staining,and the sequence of the pathogen was obtained by PCR amplification and 16S rRNA gene sequencing.The phylogenetic tree was constructed by MEGA software.Then the name of the bacterial species was identified through physiological and biochemical analysis.The results showed that Gram staining was negative,curved,and bluntly rounded at both ends;the bacterium was 1-1.5μm in length,1-1.2μm in width,with mycelium and flagellum;16S rRNA gene was 1395 bp;the establishment of the phylogenetic tree revealed that P1 was in the same branch with Vibrio cholerae;the results of physiological and biochemical experi⁃ments were in agreement with those of Vibrio cholerae,which leads to the identification of the strain as Vibrio cholerae.The results of drug sensitivity test showed that the isolated strain was highly sensitive to nine antibiot⁃ics,including cefoperazone,canomyces and vancomycin,moderate sensitive to tetracycline,resistant to 18 antibiot⁃ics,including furazolidone,cotrimoxazole and clindamycin.The results provide reference for the pathogen identifi⁃cation and drug control of P.dabryanus ssp..

关 键 词：台湾泥鳅 腐皮病 霍乱弧菌 分离 鉴定 

分 类 号：S942.9[农业科学—水产养殖]