卷柏总黄酮的纯化工艺与细胞保护作用  

Purification Process of Total Flavonoids from Selaginella pulvinata and Protecting Effects on Neuroblastoma Cells

作　　者：吕雪峰 王莲萍 张明天 张莲珠 SONG Yu LYU Xuefeng;WANG Lianping;ZHANG Mingtian;SONG Yu;ZHANG Lianzhu(Jilin Province Product Quality Supervision and Inspection Institute,Changchun 130103,China;Chinese Medicine College,Jilin Agricultural Science and Technology University,Changchun132101,China;Affiliated Hospital to Changchun Chinese Medicine University,Changchun 130021,China;College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources,Ministry of Education,Hainan Tropical Ocean University,Sanya Hainan 572022,China;Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Hainan Tropical Ocean University,Sanya Hainan 572022,China)

机构地区：[1]吉林省产品质量监督检验院,长春130103 [2]吉林农业科技学院中药学院,长春132101 [3]长春中医药大学附属医院,长春130021 [4]海南热带海洋学院水产与生命学院,海南三亚572022 [5]海南热带海洋学院热带海洋生物资源利用与保护教育部重点实验室,海南三亚572022 [6]海南热带海洋学院海南省热带海洋渔业资源保护与利用重点实验室,海南三亚572022

出　　处：《海南热带海洋学院学报》2024年第2期104-112,共9页Journal of Hainan Tropical Ocean University

基　　金：海南省自然科学基金高层次人才项目(821RC598)。

摘　　要：卷柏(Selaginella tamariscina)含有丰富的黄酮类成分,以双黄酮为主。利用AB-8大孔树脂对脂溶性成分的吸附作用和聚酰胺树脂对黄酮类成分吸附和解吸的特点,将二者串联对卷柏提取物进行分离纯化,建立垫状卷柏总黄酮(Total flavonoids of selaginella pulvinata,TFSP)提取纯化工艺。采用紫外分光光度法测定TFSP含量,并通过MTT法,检测TFSP对神经细胞过氧化氢损伤的保护与修复作用。结果表明,垫状卷柏提取物先通过AB-8大孔树脂吸附脂溶性成分,再经聚酰胺吸附和解吸卷柏总黄酮,在聚酰胺树脂上样量1∶2(卷柏与树脂的体积比)、80%乙醇洗脱5∶1(洗脱液与聚酰胺柱的体积比)等最佳纯化工艺条件下,TFSP的平均含量达81.4%,工艺过程科学合理、简便易行。将TFSP与SH-SY5Y细胞共孵育,可显著减轻神经细胞被过氧化氢损伤的程度,并对已经受损的细胞具有明显的修复作用。TFSP具有为神经退行性疾病开发治疗药物的潜力。s:Flavonoids,especially biflavonoids,are main components of Selaginella tamariscina and Selaginella pulvinate.The purification process of total flavonoids from Selaginella pulvinata(TFSP)was established by purifying the Selaginella pulvinata extract through AB-8 macroporous resin followed by polyamide.The content of TFSP was determined by ultravio⁃let spectrophotometry.The protecting and repairing effects of TFSP on hydrogen peroxide induced neuron cell damage were detected by MTT.The results showed that AB-8 macroporous resin adsorbed and removed the liposoluble substance from the extract,and flavonoids were isolated through adsorption-desorption in polyamide column.The content of TFSP yielded from the process was up to 81.4%under the optimal conditions—polyamide resin uploading volume of 1∶2(C.matta vs.resin),and 80%ethanol eluent of 5∶1(eluent vs.polyamide column).The purification process is scientific,reasonable,simple and easy to implement.TFSP showed remarkable activity on neuroblastoma cell and increased the cell viability before or after it was cocultured with SH⁃SY5Y cells.Therefore,TFSP has good effects on nerve cell peroxidation damage with the potential to be developed as therapeutic drug for neurodegenerative disorder.

关 键 词：卷柏总黄酮 纯化 大孔树脂 神经细胞 保护作用 

分 类 号：R28[医药卫生—中药学]