微滴式数字PCR技术在动物疫病检测中的应用研究进展  

Research Progress in the Application of Droplet-based Digital PCR Assay to Animal Disease Detection

作　　者：冯杰 聂洪波 曹乾大 朱坤华 敖元富 黎春秀 Feng Jie;Nie Hongbo;Cao Qianda;Zhu Kunhua;Ao Yuanfu;Li Chunxiu(Fushun Animal Husbandry and Veterinary Technology Extension Center,Fushun 643200,Sichuan,China;Fushun Animal Disease Control Center,Fushun 643200,Sichuan,China;Fushun Agricultural Comprehensive Management Station,Fushun 643200,Sichuan,China)

机构地区：[1]富顺县畜牧兽医技术推广中心,四川富顺643200 [2]富顺县动物疫病预防控制中心,四川富顺643200 [3]富顺县农业综合管理站,四川富顺643200

出　　处：《中国动物检疫》2024年第4期58-63,90,共7页China Animal Health Inspection

摘　　要：数字PCR(dPCR)技术是最近发展起来的第三代PCR技术,其中微滴式数字PCR(ddPCR)技术具有敏感性高、耐受性强,准确性和重复性好,可实现不依赖标准曲线即可对样本目标核酸进行绝对定量的优点,得到研究者的广泛关注。ddPCR技术凭借诸多优势,近年来在动物疫病病原检测领域得到了飞速发展,已被成功应用于病毒、细菌、寄生虫以及支原体、衣原体等多种病原的检测,尤其为低丰度病原调查、实验室检测和病原分型鉴定提供了强有力的技术支撑。本文综述了ddPCR技术的基本原理及其在动物疫病诊断中的应用和发展前景,以期为ddPCR技术在兽医实验室动物疫病诊断方面的推广提供参考。Digital PCR(dPCR)is the third generation PCR developed recently,especially droplet-based digital PCR(ddPCR),with the advantages of high sensitivity,high tolerance,good accuracy,good reproducibility as well as absolute quantification for target nucleic acids in sample without relying on the standard curve,has been widely concerned by researchers.With a number of advantages,ddPCR has been rapidly developed in the field of animal disease pathogen detection,and successfully used to detect viruses,bacteria,parasites,Mycoplasma,Chlamydia and other pathogens,especially supporting the investigation into pathogens at low abundance,laboratory testing and typing identification.In the study,the general principles of ddPCR as well as its application and prospects in animal disease diagnosis were reviewed,with a view to providing a reference for application of the technology to animal disease diagnosis in veterinary laboratories.

关 键 词：微滴式数字PCR 绝对定量 兽医实验室 动物疫病诊断 

分 类 号：S851.3[农业科学—预防兽医学]