蒙药光明盐四味汤散HPLC指纹图谱及2个成分含量测定研究  

Study on HPLC Fingerprint and Content Determination of Two Components of Mongolian Medicine Guangmingyan Siwei Tangsan

作　　者：于智远 田向阳 何春龙 YU Zhi-yuan;TIAN Xiang-yang;HE Chun-ong(Department of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《中国民族医药杂志》2024年第1期44-47,共4页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古医科大学大学生科技创新“英才培育”项目(YCPY2022041);内蒙古医科大学2022年学科建设项目(YKD2022XK011)。

摘　　要：目的:建立光明盐四味汤散HPLC指纹图谱,测定没食子酸和胡椒碱含量,提高光明盐四味汤散的质量标准。方法:采用HPLC法,色谱柱为Agilent 5 TC-C_(18)(4.6 mm×250 mm);流动相为0.1%磷酸水(A)-甲醇(B)梯度洗脱;流速为1 mL·min^(-1);紫外检测波长为300 nm;柱温为30℃;进样量为10 mL。结果:10批样品的HPLC图确定11个共有峰,计算相似度为0.995以上,3号峰为没食子酸,10号峰为胡椒碱。没食子酸在21.76~130.56范围内呈良好的线性关系,r=0.9997;胡椒碱在105.1~420.4平均加样回收率分别为98.73%和99.26%,RSD小于2%。结论:该方法简便、准确,可用于光明盐四味汤散的质量控制,提高该药质量标准。Objective:To establish the HPLC fingerprint of Guangmingyan Siwei Tangsan,determine the content of Gallic acid and Piperine,and improve the quality standard of Guangmingyan Siwei Tangsan.Method:HPLC method was used,with Agilent 5 TC-C_(18)(4.6 mm×250 mm)as the chromatographic column;The mobile phase is 0.1%phosphoric acid water(A)-methanol(B)gradient elution;The flow rate is 1 mL·min^(-1);The UV detection wavelength is 300 nm;The column temperature is 30℃;Injection volume is 10μL.Result:A total of 11 common peaks were identified in the HPLC plots of 10 batches of samples,with a calculated similarity of over 0.995.The No.3 peak is Gallic acid,and the No.10 peak is Piperine.Gallic acid in 21.76μg·mL^(-1)-130.56μg·mL^(-1)range,There is a good linear relationship,r=0.9997;Piperine in 105.1μg·mL^(-1)-420.4μg·mL^(-1)range,There is a good linear relationship,r=0.9990;The average sample recovery rates were 98.73%and99.26%,respectively,with RSD less than 2%.Conclu sion:This method is simple and accurate,and can be used for the quality control of Guangmingyan Siwei Tangsan,improving the quality standard of the drug.

关 键 词：光明盐四味汤散 指纹图谱 高效液相色谱法 质量控制 含量测定 

分 类 号：R291.2[医药卫生—民族医学]