藜麦热激蛋白60(CqHSP60)基因家族生物信息学和表达模式分析  

Bioinformatics and Expression Pattern Analysis of Heat Shock Proteins 60 Gene(CqHSP60)Family of Quinoa(Chenopodium quinoa Willd.)

作　　者：陈方军 刘文莉 杨博 彭悦 丁洪霞 宫瑗 尹海波[1] 郭善利[1] CHEN Fangjun;LIU Wenli;YANG Bo;PENG Yue;DING Hongxia;GONG Yuan;YIN Haibo;GUO Shanli(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2024年第2期155-162,共8页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(31370296);山东省“渤海粮仓”科技示范工程升级版计划项(2019BHLC001);烟台市校地融合发展项目。

摘　　要：为了探究藜麦HSP60基因(CqHSP60)的结构特征、基因功能和表达模式,基于藜麦全基因组和转录组数据,利用生物信息学方法对CqHSP60基因家族进行鉴定和系统分析。依据亚细胞结构定位分为了三个亚组,每个亚组具有相似的Motif和基因结构;通过RNA-Seq数据对CqHSP60基因在不同发育阶段进行了表达分析,并对在热、干旱、盐胁迫等不同处理条件下CqHSP60基因的胁迫响应进行了分析,结果表明CqHSP60基因在花簇和干种子中表达量较高,并对不同的胁迫模式均有响应;在CqHSP60基因的启动子区发现了多个与胁迫相关的顺式调控元件,这表明这些基因在多重胁迫下受到应激响应。In order to explore the structural characteristics,gene function and expression pattern of quinoaHSP60 gene(CqHSP60),the CqHSP60 gene family is identified and systematically analyzed by using bioinformatics methods.The result showes that 37 CqHSP60 genes are identified in the quinoa genome,which can be divided into 3 phylogenetic subgroups according to subcellular structure localization,and each subgroup has similar motif and gene structure.Through RNA-Seq data,the gene expression of tissues at different developmental stages and different treatments such as heat,drought and salt stress are analyzed.CqHSP60 genes respond to different stress,and express higher in flower clusters and dried seeds.Multiple stress-associated cis-regulatory elements are identified in the promoter region of the CqHSP60 gene,suggesting that these genes are subjected to stress responses under multiple stresses.

关 键 词：藜麦 HSP60 表达模式 胁迫 伴侣蛋白 

分 类 号：Q751[生物学—分子生物学]