基于16S rRNA测序的1型和2型糖尿病大鼠肠道菌群差异  

Difference of Gut Microbiota between Type 1 and Type 2 Diabetes Rats Based on 16S rRNA Gene Sequencing

作　　者：倪妍 滕新栋 徐丽 王绪敏 曲江勇[1] 王丽君 金黎明 邢志凯 刘秀梅 NI Yan;TENG Xindong;XU Li;WANG Xumin;QU Jiangyong;WANG Lijun;JIN Liming;XING Zhikai;LIU Xiumei(School of Life Sciences,Yantai University,Yantai 264005,China;Qingdao International Travel Healthcare Center,Qingdao 266071,China;Qingdao Dagang customs,Qingdao 266071,China;Key Laboratory of Biotechnology and Bioresources Utilization,Dalian Minzu University,Dalian 116600,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]青岛国际旅行卫生保健中心,山东青岛266071 [3]青岛大港海关,山东青岛266071 [4]大连民族大学生物技术与生物资源利用重点实验室,辽宁大连116600

出　　处：《烟台大学学报（自然科学与工程版）》2024年第2期163-171,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：生物技术与资源利用教育部重点实验室(大连民族大学)开放基金(KF2022006);烟台“双百人才项目”(2320004-SM20RC02)。

摘　　要：通过16S rRNA测序分析T1DM与T2DM大鼠肠道菌群的差异。Alpha多样性分析显示,T1DM组和T2DM组肠道菌群多样性和均匀度均高于对照组,Beta多样性分析显示不同组样本间存在差异且有很好的聚类。在门水平上,T1DM和T2DM大鼠肠道菌群以Firmicutes和Bacteroidetes为主,Firmicutes/Bacteroidetes(F/B)比例相比于对照组增加,且T2DM组高于T1DM组。与对照组相比,T1DM组Actinobacteria丰度显著增加,Anaerofustis丰度显著降低。在T2DM大鼠中,Proteobacteria、Oscillospira丰度显著增加。Oscillospira丰度在T2DM组增加,在T1DM组无明显差异。Rhodococcus为T1DM组的特有属,Coprobacillus和Roseburia为T2DM组的特有属。研究结果表明大鼠肠道菌群失调与T1DM和T2DM密切相关,可为T1DM和T2DM患者的个性化治疗提供科学依据。Difference between T1DM and T2DM rat gut microflora is analyzed through 16S rRNA gene sequencing.Alpha diversity analysis show that the diversity and evenness of intestinal flora in the T1DM and T2DM groups are higher than those in the control group,and Beta diversity analysis show that there are differences and good clustering among different groups.At the phylum level,the gut microbiota of T1DM and T2DM are dominate by Firmicutes and Bacteroidetes.The proportion of Firmicutes/Bacteroidetes(F/B)increases in T1DM and T2DM groups and the ratio in T2DM is higher than T1DM.In T1DM group the abundance of Actinobacteria increases and the abundance of Anaerofustis decreases significantly compared with the control group.The abundance of Proteobacteria and Oscillospira increase significantly in T2DM rats.T2DM group shows an increased abundance of Oscillospira,while the contrast is not significant in T1DM group.Rhodococcus is the unique genus in T1DM group,Coprobacillus and Roseburia are the unique genera in T2DM rats.The results show that the imbalance of rat gut microflora is closely related to T1DM and T2DM,Which may provide scientific basis for individualized treatment of T1DM and T2DM.

关 键 词：肠道菌群 T1DM T2DM 16S rRNA测序 

分 类 号：Q939[生物学—微生物学]