不同烤烟品种根际微生物多样性群落结构分析  

Analysis on Rhizosphere Microbial Diversity and Community Structure of Different Flue-cured Tobacco Varieties

作　　者：何晓冰 黄昆鹏 李俊营 马世原 张鹏哲 王明鑫[1] 杨楠[1] 阴广宇 张富生 孙聚涛 HE Xiaobing;HUANG Kunpeng;LI Junying;MA Shiyuan;ZHANG Pengzhe;WANG Mingxin;YANG Nan;YIN Guangyu;ZHANG Fusheng;SUN Jutao(Pingdingshan Branch,Henan Tobacco Company,Pingdingshan,Henan 467000;College of Tobacco Sciences,Henan Agricultural University,Zhengzhou,Henan 450000,China)

机构地区：[1]河南省烟草公司平顶山市公司,河南平顶山467000 [2]河南农业大学烟草学院,河南郑州450000

出　　处：《贵州农业科学》2024年第4期56-65,共10页Guizhou Agricultural Sciences

基　　金：中国烟草总公司河南省公司重点项目(2021410000240021);河南省烟草公司许昌市公司重点项目(2020411000240069);河南省烟草公司平顶山市公司重点项目(2022.41.04.01.24.0105)。

摘　　要：【目的】分析不同烤烟品种植株根际土壤细菌及真菌群落结构,探明烤烟植株与其根际微生物的关系,为改良烟株根际微生态环境,促进烟草生产中的病虫害防治,提高烟叶品质提供理论依据。【方法】以中烟100、NC89和云烟87为试验材料,利用Illumina高通量技术,结合植株根际土壤理化性质分析不同品种烤烟植株间根际土壤微生物群落组成和多样性的差异。【结果】烟草根际微生物群落中,根际土壤细菌4个优势菌门为放线菌门、变形菌门、酸杆菌门和绿弯菌门;根际土壤真菌2个优势菌门为子囊菌门和被孢霉门。品种NC89的Alpha多样性显著高于中烟100和云烟87,说明NC89细菌多样性优于其他2个品种,有益菌Mortierella、Neocosmospora、unclassified_k_Fungi的含量高于其他2个品种。【结论】不同烤烟品种植株根际土壤中细菌群落和真菌群落的组成及多样性存在显著差异,NC89根际土壤细菌和真菌的多样性群落结构优于中烟100和云烟87。【Objective】The rhizosphere bacterial community and fungal community structure of different flue-cured tobacco varieties were explored,and the relationship between flue-cured tobacco plants and rhizosphere microorganisms was studied,which provided theoretical basis for improving rhizosphere microecological environment of tobacco plants,promoting disease and pest control during tobacco production and improving tobacco quality.【Method】Using Zhongyan 100,NC89 and Yunyan 87 as experimental materials,Illumina high-throughput technology combined with the physicochemical properties of rhizosphere soil were used to analyze the differences of rhizosphere soil microbial community composition and diversity among different varieties of flue-cured tobacco plants.【Result】In the rhizosphere microbial community of tobacco,the four dominant phyla of rhizosphere soil bacteria were Actinobacteriota,Proteobacteria,Acidobacteriota and Chloroflexi.The two dominant phyla of rhizosphere soil fungi were Ascomycota and Mortierellomycota.The alpha diversity of cultivar NC89 was significantly higher than Zhongyan 100 and Yunyan 87,showing that NC89 bacteria diversity was superior to other two varieties,and the beneficial bacteria Mortierella,Neocosmospora,unclassified_k_Fungi content was higher than the other two varieties.【Conclusion】There are significant differences in the composition and diversity of bacterial and fungal communities in the rhizosphere soil of different fluecured tobacco varieties.The diversity of bacterial and fungal communities in the rhizosphere soil of NC89 is better than that of Zhongyan 100 and Yunyan 87.

关 键 词：烟草 品种 根际微生物 多样性 高通量测序 群落结构 

分 类 号：S516[农业科学—作物学]