构建血管内皮因子修饰的组织工程尿道修复大鼠尿道缺损  

Construction of Tissue Engineering Urethra Modified with Vascular Endothelial Factor for Repairing Urethral Defects in Rats

作　　者：李航旭 刘小燕 Li Hangxu;Liu Xiaoyan(Department of Urology,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China;Beijing Garrison Fengtai Tenth Retired Cadre Rehabilitation Center,Beijing 100071 China)

机构地区：[1]锦州医科大学附属第三医院泌尿外科,辽宁锦州121000 [2]北京卫戍区丰台第十离职干部休养所,北京100071

出　　处：《锦州医科大学学报》2024年第2期51-55,共5页Journal of Jinzhou Medical University

摘　　要：目的探讨血管内皮因子修饰的组织工程尿道修复大鼠尿道缺损的效果。方法取SD大鼠尿道,共20根,每段长1.5 cm,采用化学去细胞法处理大鼠尿道的细胞成分,保留细胞外基质,制备组织工程尿道。将10只Wistar大鼠尿道制成1.5 cm整段缺损模型,用SD大鼠经过血管内皮因子修饰的组织工程尿道修复(处理组)。将10只Wistar大鼠尿道制成1.5 cm整段缺损模型,用SD大鼠去细胞同种异体尿道移植修复Wistar大鼠尿道的整段缺损(对照组)。结果处理组与对照组相比VG染色结果显示,处理组尿道吻合口断端被染成红色,肌纤维排列整齐分布均一,大量再生肌纤维长入远端尿道。对照组吻合口肌纤维形态均一,肌纤维排列整齐分布均一,可见少量肌纤维长入远端尿道。HE染色结果处理组可见大小不等血管长入去细胞尿道内,部分有形成血窦的倾向;而对照组未见尿道被吸收,可见少量血管形成倾向。电生理检测结果测电极刺激S1神经。处理组:潜伏期(1.62±0.47)ms,波幅(1.11±0.67)mv。对照组:潜伏期(2.31±0.17)ms,波幅(2.51±0.23)mv。经过血管内皮因子修饰的组织工程尿道具有免疫原性弱、细胞膜残片去除完全、尿道基底膜保存完好等优点。结论以鼠的脱细胞尿道为基质,构建组织工程尿道,可以修复1.5 cm长的鼠尿道整段缺损,并可降低并发症的发生率,且术后能获得良好的粘膜覆盖,形成类似于正常尿道的组织结构。Objective To investigate the effect of vascular endothelial factor modified tissue engineering urethra on repairing urethral defects in rats.Methods A total of 20 SD rat urethra segments,each 1.5 cm long,were taken.The cellular components of the rat urethra were treated with chemical acellular method,while the extracellular matrix was preserved to prepare tissue engineered urethra.Ten Wistar rats were used to create a 1.5 cm complete segment defect model of the urethra,and SD rats were repaired with tissue engineering urethra modified with vascular endothelial factor(treatment group).Ten Wistar rats were used to create a 1.5 cm complete segment defect model of the urethra,and SD rat acellular allogeneic urethral transplantation was used to repair the entire segment defect of the Wistar rat urethra(control group).Results Compared with the control group,the VG staining results showed that the broken end of the urethral anastomosis in the treatment group was dyed red,and the muscle fibers were arranged neatly and evenly,with a large amount of regenerated muscle fibers growing into the distal urethra.The morphology of the anastomotic muscle fibers in the control group is uniform,and the muscle fibers are arranged neatly and evenly distributed.A small amount of muscle fibers can be seen growing into the distal urethra.The HE staining results showed that blood vessels of varying sizes grew into the acellular urethra in the treatment group,with some tending to form blood sinuses;however,the control group did not show any absorption of the urethra or tendency towards angiogenesis.The electrophysiological test results showed that the electrode stimulated the S1 nerve.Treatment group:Latency(1.62±0.47)ms,amplitude(1.11±0.67)mv.Control group:Latency(2.31±0.17)ms,amplitude(2.51±0.23)mv.The tissue engineering urethra modified with vascular endothelial factor has the advantages of weak immunogenicity,complete removal of cell membrane fragments,and intact preservation of the urethral basement membrane.Conclusion Using the a

关 键 词：血管内皮因子 尿道缺损 鼠 组织工程尿道 

分 类 号：R699.6[医药卫生—泌尿科学]