环糊精强化镰刀菌修复土壤污染生态环境中高环多环芳烃的研究  

Research on cyclodextrin enhanced Fusarium remediation of soil contaminated ecological environment with HMW-PAHs

作　　者：张萌 祁明月 张光伟 高佳圆 李家辉 王小敏[1] 宁国辉[1] 杨志新[1,2] ZHANG Meng;QI Mingyue;ZHANG Guangwei;GAO Jiayuan;LI Jiahui;WANG Xiaomin;NING Guohui;YANG Zhixin(College of Resource and Environmental Sciences,Hebei Agricultural University,Baoding 071000,China;State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071000,China)

机构地区：[1]河北农业大学资源与环境科学学院,河北保定071000 [2]省部共建华北作物改良与调控国家重点实验室,河北保定071000

出　　处：《林业与生态科学》2024年第2期207-214,共8页Forestry and Ecological Sciences

基　　金：河北省教育厅重点项目(ZD2021057);华北作物改良与调控国家重点实验室课题(NCCIR2022ZZ-9);中央引导地方科技发展资金项目(226Z7301G);现代农业产业技术体系河北省创新团队建设(HBCT2024140209)。

摘　　要：以煤矿区高环芳烃(HMW-PAHs)污染土壤为研究对象,通过添加环糊精且接种镰刀菌ZH-H2试验,研究镰刀菌ZH-H2处理、环糊精处理及二者组合处理对污染老化土壤5环和6环6种单体PAHs的修复效果。结果表明,镰刀菌单一处理对5环和6环PAHs的去除率在14.37%~22.85%之间;在不同剂量环糊精处理下(J1、J2、J3),J2处理(1 g/kg)对6种HMW-PAHs单体的去除率表现最高,为13.51%~28.43%;在ZH-H2-环糊精2因素处理中,H+J3处理对6种HMW-PAHs单体(BbF、BkF、BaP、DbA、InP、BghiP)及总量的去除效果最佳,其去除率分别为24.61%、25.15%、23.14%、34.18%、25.76%、23.46%、25.29%,比ZH-H2单一处理分别提高了71.33%、66.68%、28.94%、49.58%、44.94%、55.88%、60.71%(P<0.05)。就5环HMW-PAHs去除率,H+J3处理显著高于H+J1处理,H+J2与H+J3之间差异不显著;针对6环HMW-PAHs去除率,H+J3处理显著高于H+J1、H+J2处理。从土壤酶活性来看,ZH-H2-环糊精2因素处理下土壤木质素过氧化物酶活性均显著高于其他处理,且与5环、6环芳烃去除率大体上呈现显著正相关关系。综合来看,ZH-H2(1 g/kg)-环糊精(5 g/kg)处理是高效修复5~6环芳烃的优势组合。The soil contaminated by high cyclic aromatic hydrocarbons(HMW-PAHs)in coal mine area was taken as the research object.Remediation effects of Fusarium ZH-H2 treatment,cyclodextrin treatment and a combination of the two on 6 monomeric PAHs in 5-ring and 6-ring contaminated soil were studied by adding cyclodextrin and inoculating Fusarium ZH-H2.The results showed that the removal rates of 5-ring and 6-ring PAHs by Fusarium were 14.37%22.85%,and the removal rate of 6 HMW-PAHs monomers were the highest under different doses of cyclodextrin treatment(J1,J2,J3),J2 treatment(1 g/kg)was the highest,which was 13.51%28.43%,and in the ZH-H2-cyclodextrin treatment,H+J3 treatment had the best removal effect on 6 HMW-PAHs monomers(BbF,BkF,BaP,DbA,InP,BghiP)and the total amount,with removal rates of 24.61%,25.15%,23.14%,34.18%,25.76%,23.46%and 25.29%,respectively,which were 71.33%,66.68%,28.94%,49.58%,44.94%,55.88%and 60.71%higher than those of ZH-H2 single treatment(P<0.05).The removal rate of 5-ring HMW-PAHs was significantly higher in H+J3 treatment than in H+J1 treatment,and there was no significant difference between H+J2 and H+J3,while in terms of removal rate of 6-ring HMW-PAHs,H+J3 treatment was significantly higher than that in H+J1 and H+J2 treatments.From the perspective of soil enzyme activity,the activity of soil lignin peroxide under ZH-H2-cyclodextrin treatment was significantly higher than that of other treatments,and there was a significant positive correlation with the removal rate of 5-ring and 6-ring HMW-PAHs.On the whole,ZH-H2(1 g/kg)-cyclodextrin(5 g/kg)treatment was an advantageous combination for efficient treatment of 5-6 ring HMW-PAHs.

关 键 词：环糊精 镰刀菌 高环多环芳烃 去除率 酶活性 

分 类 号：X53[环境科学与工程—环境工程]