基于ITS序列对两株野生大型真菌的鉴定及系统发育分析  

Identification and Phylogenetic Analysis of Two Wild Macrofungi Using ITS Sequences

作　　者：李春霖 肖强 张瑛[2] 万松胜 李双龙 Li Chunlin;Xiao Qiang;Zhang Ying;Wan Songsheng;Li Shuanglong(School of Forestry and Horticulture,Hubei Minzu University,Enshi 445000;Enshi Tujia Minority and Miao Minority Autonomous Prefecture Academy of Forestry,Enshi 445000;Key Laboratory of Biologic Resources Protection and Vtilization of Hubei Provice,Enshi 445000)

机构地区：[1]湖北民族大学林学园艺学院,恩施445000 [2]恩施州林业科学研究院,恩施445000 [3]生物资源保护与利用湖北省重点实验室,恩施445000

出　　处：《湖北林业科技》2024年第2期46-50,共5页Hubei Forestry Science and Technology

基　　金：恩施州科技计划青年人才类项目“恩施市大型真菌资源调查”(XYJ2022000067);恩施州“启航专项”科技计划项目“恩施州鲜艳乳菇种质资源收集及评价”(D20230017);中央财政林业科技推广示范项目“武陵山区林下经济模式推广示范”(鄂[2022]TG号)。

摘　　要：为进一步确定菌种鉴定的准确性和科学性,通过采用传统形态学分类法与ITS序列分析法对采自湖北省武陵山地区恩施市境内的2株野生真菌进行分类鉴定与系统发育分析。结果表明:依据其形态特征初步鉴定该2株菌株为绒盖牛肝菌属菌种;对2株菌株样本的ITS全区序列进行PCR扩增测序,并在NCBI数据库中进行Blast比对,采用NJ邻接法构建出系统发育树,通过探究其与已知菌种的相关性进行分析鉴定。确定样本HBES1003为绒盖条孢牛肝菌,样本HBES1096为黑斑绒盖牛肝菌。In order to further determine accuracy and scientific nature of identification,the company classified and scribed two wild fungus collected from Enshi,Hubei Province,and analyzed their phylogeny by using traditional morphological classification and ITS(Internal transcribed spacer)sequences analysis.The results showed that two strains were identified as Xerocomus species based on their morphological characteristics.PCR amplification and sequencing of ITS whole region sequences of the samples of the two strains were performed,Blast comparison was performed in NCBI database,and the phylogenetic tree was constructed by NJ(Neighbor-Joining)adjacency method.It was analyzed and identified by exploring its correlation with known strains.sample HBES1003 was Xerocomus subtomentosus and sample HBES1096 was X.nigromaculatus.

关 键 词：野生真菌 ITS序列 系统地位 

分 类 号：Q938.2[生物学—微生物学]