基于3DEEM-PARAFAC方法对不同秸秆还田方式下WSOC的组分与荧光特征分析  

Analysis of Composition Fluorescence Characteristics of SWOC in Different Straw Returning Modes Based on 3DEEM-PARAFAC Method

作　　者：王楠楠 孙冬梅[2] 李玉梅 Wang Nannan;Sun Dongmei;Li Yumei(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Life Science and Technology,Heilongjiang Bayi Agricultural University;Heilongjiang Institute of Black Soil Protection and Utilization)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319 [2]黑龙江八一农垦大学生命科学技术学院 [3]黑龙江省黑土保护利用研究院

出　　处：《黑龙江八一农垦大学学报》2024年第2期15-22,共8页journal of heilongjiang bayi agricultural university

基　　金：国家重点研发计划(2022YFD1500305);国家大豆产业技术体系(CARS-0401A)。

摘　　要：以三维荧光光谱结合平行因子法(3DEEM-PARAFAC)为主要分析方法,分析秸秆未还田条件下3种耕作方法(免耕、浅翻、深翻)及相应还田方法(秸秆免耕覆盖、浅翻还田、深翻还田)下,土壤水溶性有机碳(WSOC)的组分及不同土层的荧光特性。结果表明,土壤WSOC解析出4个荧光组分,分别为2个类腐殖质物质C1(310,240/410 nm)、C2(270/465 nm)和两个类蛋白物质C3(280,230/340 nm)、C4(220/300 nm);秸秆深翻还田在>35 cm土层有不同程度的促进类腐殖华物质C1、C2组分向类蛋白物质C4组分转化的趋势;土壤WSOC的来源均为植物源与微生物源的混合贡献,深翻还田在>35 cm土层腐殖化程度最低。秸秆还田与深翻对深层土壤WSOC中结构较为复杂、芳香性高的有机物的矿化分解有一定促进作用。Three dimensional fluorescence spectroscopy combined with parallel factor method(3DEEM-PARAFAC)was used to analyze the composition of water-soluble organic carbon(WSOC)in soil and the fluorescence characteristics of different soil layers under three tillage methods(no-tillage,shallow tilling,deep tilling)and corresponding tillage methods(straw no-tillage mulding,shallow tilling,deep tilling).The results showed that four fluorescence components were determined by WSOC,namely two humic substances C1(310,240/410 nm)and C2(270/465 nm)and two protein-like substances C3(280,230/340 nm)and C4(220/300 nm).The conversion of humic substances C1 and C2 to protein-like substances C4 in the soil layer>35 cm was promoted.The source of soil WSOC was the mixed contribution of plant and microbial sources,and the humification degree was the lowest in the soil layer>35 cm.Straw returning and deep turning could promote the mineralization and decomposition of organic matter with complex structure and high aromatic properties in deep soil WSOC.

关 键 词：水溶性有机碳(WSOC) 耕作方法 秸秆还田 3DEEM-PARAFAC 

分 类 号：S153.6[农业科学—土壤学]