篦子三尖杉的生物碱成分及其抗肿瘤活性研究  

Alkaloids from Cephalotaxus oliveri Mast. and its Antitumor Activities

作　　者：姜春宇 高贵文 王璇 马高鹏 孙雯 谈佳沂 贾桂燕[1] 华会明[2] 韩通 赵蕊[1] Jiang Chunyu;Gao Guiwen;Wang Xuan;Ma Gaopeng;Sun Wen;Tan Jiayi;Jia Guiyan;Hua Huiming;Han Tong;Zhao Rui(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]沈阳药科大学中药学院

出　　处：《黑龙江八一农垦大学学报》2024年第2期84-89,126,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学2022年度定向培养(引进人才)科研启动基金项目(XYB202212);黑龙江省大学生创新训练项目(202210223093);黑龙江八一农垦大学学成引进人才科研启动计划(XYB201814);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2020041)。

摘　　要：为研究篦子三尖杉的化学成分,利用多种色谱分离技术和波谱手段,从其乙醇提取物中分离鉴定了11个生物碱类成分。其中博落回碱(7)、(2Z)-1,2-二氢-2-(1H-吲哚-3-亚甲基)-3H-吲哚-3-酮(8)、1-甲氧基-3-吲哚乙腈(9)、4-甲氧基-1H-吲哚-3-甲醛(10)、吲哚-3-甲醛(11)为首次从三尖杉属植物中分离得到。采用CCK-8法测试了化合物1~11对人乳腺癌细胞株MCF-7、人肝癌细胞株HepG2和人肺癌细胞株A549的体外抗增殖能力,异三尖杉酯碱(1)和三尖杉酯碱(2)对三种肿瘤细胞显示了显著的抗增殖活性。To study the chemical constituents of Cephalotaxus oliveri Mast.,eleven alkaloids were isolated and identified from its ethanol extract by various chromatographic and spectral methods.Bocconoline(7),(2Z)-1,2-dihydro-2-(1H-indol-3-ylmethylene)-3H-indol-3-one(8),1-methoxy-3-indoleacetonitrile(9),4-methoxy-1H-indole-3-carbaldehyde(10)and indole-3-carboxaldehyde(11)were isolated from Cephalotaxus genus for the first time.The antiproliferative effects of compounds 1~11 against human breast cancer cell line MCF-7,human liver cancer cell line HepG2 and human lung cancer cell line A549 in vitro were assayed by CCK-8 method,among them,isoharringtonine(1)and harringtonine(2)showed significant antiproliferative activities.

关 键 词：篦子三尖杉 生物碱 抗肿瘤活性 

分 类 号：Q78[生物学—分子生物学]