长白猪和藏猪在不同日龄、种间的代谢物差异分析  

Analysis of Metabolite Differences Between Landrace and Tibetan Pigs at Different Days of Age and between Species

作　　者：李江凌[1,2] 张金灵 陈晓晖[1,2] 王秋实 赵素君[1,2] 刘锐 吕学斌[1,2] 王宇萍 龚建军[1,2] 何志平 LI Jiangling;ZHANG Jinling;CHEN Xiaohui;WANG Qiushi;ZHAO Sujun;LIU Rui;LV Xuebin;WANG Yuping;GONG Jianjun;HE Zhiping(Sichuan Animal Science Academy,Chengdu 610066,China;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Chengdu 610066,China;Sichuan Animal Feed General Station,Chengdu 610066,China)

机构地区：[1]四川省畜牧科学研究院,四川成都610066 [2]动物遗传育种四川省重点实验室,四川成都610066 [3]四川省饲料工作总站,四川成都610041

出　　处：《中国猪业》2024年第2期24-34,共11页China Swine Industry

基　　金：四川省基本科研业务项目(SASA202306、SASA202208);四川省科技计划资助项目(2021YFYZ0009、2021YFYZ0030);四川省财政运行专项项目(SASA2024CZYX007)。

摘　　要：通过对地方品种猪及外种猪的不同生长阶段代谢组学进行比较研究,以此了解地方品种猪与外种猪代谢组差异与耐粗饲性状的关系。采用UPLC-MS技术对7日龄和30日龄的长白猪和藏猪的粪便样品开展代谢组学研究,分析代谢物的变化情况,筛选差异代谢物的情况。结果显示,长白猪不同日龄代谢物VI P≥1的总共有99种,其中38种代谢物的比例是上升的,另61种代谢物主要存在下降趋势;藏猪不同日龄代谢物VIP≥1的总共有90种,其中31种代谢物的比例是上升的,另59种代谢物存在下降趋势;7日龄的长白猪和藏猪代谢物对比发现VIP≥1的总共有59种,其中29种代谢物的比例主要是上升趋势,另30种是下降趋势;30日龄的长白猪和藏猪代谢物对比VI P≥1的总共有66种,其中32种代谢物的比例是上升的,另34种代谢物存在下降趋势。说明不同种间、日龄代谢物含量存在差异,其中同种不同日龄猪的代谢物差异最多,说明随着年龄的增长猪对粗纤维等粗饲料的消化代谢能力有所差异。通过对代谢通路的分析显示,主要存在差异的代谢通路为Metabolic pathways、ABC transporters,参与差异代谢通路的代谢物主要是有机酸、核苷酸、氨基酸等相关代谢物,说明藏猪和长白猪在耐粗饲的特性上与粗饲料对肠道的影响有一定的关联性。此研究为解释在代谢组学方面藏猪耐粗饲特性提供了一定的理论基础。In order to study the relationship between the difference of metabolome and the character of tolerance to coarseness between Landrace and Tibetan pigs,the study of metabolomics was done in different growth stages of Landrace and Tibetan pigs.UPLC-MS technique was used to study the metabolomics of 7-day-old and 30-day-old Landrace and Tibetan pigs feces samples,analyzed the changes of metabolites and screened the differential metabolites.The results showed that there were 99 kinds metabolite with variable importance in projection(VIP)≥1 at different ages of Landrace,among them,38 kinds of metabolite were increasing,while the other 61kinds metabolite were mainly decreasing.A total of 90 kinds metabolite with VIP≥1 at different days of age in Tibetan pigs.among them,31 kinds of metabolite were increasing,while the other 59 kinds metabolite showed a decreasing trend.There were a total of 59 kinds metabolite with VIP≥1 in the comparison of 7-day-old Landrace and Tibetan pig metabolites,of which the proportions of 29 kinds metabolite showed a predominantly increasing trend and the other 30 kinds showed a decreasing trend.A total of 66 kinds metabolites with VIP≥1 were compared between Landrace and Tibetan pigs at 30 days of age,including 32 kinds of metabolite were rising,the other 34 kinds metabolite were decreasing.The result showed that there were differences in metabolite contents between species and age,with the most differences in metabolites between the same species at different ages,suggested that there were differences in the ability of pigs to digest and metabolize crude feed such as crude fiber as they age.The analysis of metabolic pathways showed that the main metabolic pathways with differences were Metabolic pathways,ABC transporters and the metabolites involved in the metabolic pathways with differences were mainly organic acids,nucleotides and amino acid related metabolites,which indicated that there was a certain correlation between the roughage tolerance characteristics of Tibetan and Landrace

关 键 词：藏猪 长白猪 代谢组学 耐粗饲 

分 类 号：S828[农业科学—畜牧学] S821.61[农业科学—畜牧兽医]