荆芥和砂仁挥发油对米象熏蒸活性的协同作用及成分研究  

Synergistic Fumigant Activity Constitutents of Nepeta cataria and Amomum villosum Essential Oils Against Sitophilus oryzae

作　　者：杨悦玲 苏懿清 何洋洋 梁宗锁[2] 王芳 梁倩 Yang Yueling;Su Yiqing;He Yangyang;Liang Zongsuo;Wang Fang;Liang Qian(College of Forestry Southwest Forestry University,Kunming 650224;Shaoxing Academy of Biomedicine of Zhejiang Sci-Tech University,Shaoxing 312000)

机构地区：[1]西南林业大学林学院,昆明650224 [2]浙江理工大学绍兴生物医药研究院,绍兴312000

出　　处：《中国粮油学报》2024年第3期11-18,共8页Journal of the Chinese Cereals and Oils Association

基　　金：浙江省科技计划项目(2022C02023);云南省“兴滇英才支持计划”青年人才项目(XDYC-QNRC-2022-0226)。

摘　　要：研究20种植物挥发油对米象(Sitophilus oryzae Linnaeus)成虫的熏蒸活性以及混配的协同作用。采用水蒸气蒸馏法提取20种植物挥发油,通过气相-质谱法和化合物消除法,确定熏蒸活性和协同作用最好的2种挥发油的活性成分。结果表明:20种植物挥发油中荆芥挥发油对米象的熏蒸活性最好,LC_(50)为13.27 mg/L。砂仁挥发油表现出最大的协同作用,共毒性因子为107.14。通过化合物消除法评价荆芥挥发油和砂仁挥发油的主要活性成分分别为胡薄荷酮和(-)-4-萜品醇,对米象熏蒸活性的LC_(50)分别为7.34、8.35 mg/L,优于荆芥挥发油的熏蒸活性。胡薄荷酮和(-)-4-萜品醇按照质量比例61.13∶6.13和1∶1的比例混配,LC_(50)分别为5.91、8.29 mg/L,随着胡薄荷酮比例的增大,混剂对米象的熏蒸活性增强。研究证实了胡薄荷酮和(-)-4-萜品醇作为植物源杀虫剂具有防治米象的潜力。The fumigant activity of 20 essential oils and their mixtures against Sitophilus oryzae was examined.The essential oils were extracted by steam distillation,and the active components of the two essential oils with the best fumigation activity and synergistic activity were determined by gas chromatography-mass spectrometry and compound elimination assay methods.The results indicated that the essential oils from N.cataria exhibited the strongest fumigant toxicity against S.oryzae with the LC_(50) value of 13.27 mg/L.when the remaining oils were mixed with N.cataria oil,A.villosum essential oil showed the greatest promoting effect,and the co-toxicity factor was 107.14.Pulegone and(-)-terpinen-4-ol were determined as the major active constituents for the fumigant activity of N.cataria and A.villosum essential oils.The LC_(50) values were 7.34 and 8.35 mg/L,respectively,better than that of the nepeta cataria essential oil.Pulegone and(-)-terpinen-4-ol were mixed according to the mass ratio 61.13∶6.13 and 1∶1,with LC_(50) values of 5.91 and 8.29 mg/L,respectively.The fumigation activity of the mixture against S.oryzae increased with the increase of the proportion of pulegone.The experiment confirmed that pulegone and(-)-terpinen-4-ol as botanical insecticides have the potential to control S.oryzae.

关 键 词：荆芥挥发油 砂仁挥发油 米象 化合物消除法 混配 

分 类 号：S482.39[农业科学—农药学]