马铃薯青枯病拮抗菌的筛选及温室防治效果研究  

Screening of Antagonistic Bacteria against Potato Bacterial Wilt and Study on Its Control Effect in Greenhouse

作　　者：李盛 李翔 朱美如 王夏 李昊阳 谭欣如 王海燕[1] Li Sheng;Li Xiang;Zhu Meiru;Wang Xia;Li Haoyang;Tan Xinru;Wang Haiyan(College of Life Sciences,Sichuan University/Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education,Chengdu 610065,Sichuan,China)

机构地区：[1]四川大学生命科学学院/生物资源与生态环境教育部重点实验室,四川成都610065

出　　处：《作物杂志》2024年第2期242-248,共7页Crops

基　　金：国家自然科学基金(32060720)。

摘　　要：马铃薯青枯病是由青枯雷尔氏菌(Ralstonia solanacearum E.F. Smith)引起的毁灭性土传病害,筛选对马铃薯青枯病有良好防效的生防菌株具有重要意义。本研究从健康马铃薯、番茄、茄子和辣椒植株根际土壤中筛选鉴定出对马铃薯、番茄和烟草青枯病菌均具有较好抑制活性的6株拮抗菌,经分子生物学鉴定为赖氨酸芽胞杆菌(Lysinibacillus sp.)、假单胞菌(Pseudomonas sp.)和科萨克氏菌(Kosakonia sp.),分别具有产蛋白酶、铁载体和生物膜抑菌代谢物的能力。拮抗菌假单胞菌FC-17对“陇薯7号”和“大西洋”马铃薯青枯病的温室防治效果分别达到79.46%和70.83%,具有应用前景。Bacterial wilt of potato is a destructive soil-borne disease caused by Ralstonia solanacearum.Screening antagonistic bacteria against pathogen is a vital work for the biocontrol of potato bacterial wilt.Six isolates with good inhibitory effect on three R.solanacearum strains of potato,tomato and tobacco were isolated from the rhizosphere soil of healthy potato,tomato,eggplant and pepper plants.They were identified as Lysinibacillus sp.,Pseudomonas sp.and Kosakonia sp.through molecular detection,and it had the ability to produce protease,siderophor and biofilm,respectively.In greenhouse,strain FC-17 significantly reduced disease incidence by 79.46%and 70.83%on potato cultivars“Longshu 7”and“Atlantic”,respectively,indicating good potential for biocontrol of potato bacterial wilt.

关 键 词：马铃薯青枯病 拮抗细菌 抑菌代谢物 防治效果 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]