越婢汤对阿霉素诱导的人脐静脉内皮细胞高通透性的影响及机制研究  

Study on the Effects and Mechanism of Yuebi Decoction on Adriamycin-induced Hyperpermeability of Human Umbilical Vein Endothelial Cells

作　　者：程苏 李婷婷[1] 王艳茹 邵命海[1] CHENG Su;LI Tingting;WANG Yanru;SHAO Minghai(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,TCM Institute of Kidney Disease,Shanghai University of Traditional Chinese Medicine,Key Laboratory of Liver and Kidney Diseases,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院,上海中医药大学中医肾病研究所,上海中医药大学肝肾疾病病症教育部重点实验室,上海市中医临床重点实验室,上海201203

出　　处：《中国中医药信息杂志》2024年第5期92-98,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81973807)。

摘　　要：目的研究越婢汤对阿霉素诱导的人脐静脉血管内皮细胞(HUVEC)高通透性的影响,探讨其治疗肾病综合征水肿的可能作用机制。方法将HUVEC分为对照组、模型组和越婢汤低、中、高剂量组(200、300、400μg/mL),CCK-8法检测细胞活力,Transwell法检测细胞通透性,Western blot检测凋亡信号调节激酶1(ASK1)、闭锁小带蛋白-1(ZO-1)、血管内皮-钙黏蛋白(VE-cadherin)、p38蛋白表达,免疫荧光法检测ZO-1和VE-cadherin表达。结果与对照组比较,模型组HUVEC细胞活力明显降低,细胞通透性明显增加,细胞肿胀、膜结构破坏,ASK1、p-p38蛋白表达明显升高,ZO-1、VE-cadherin蛋白表达明显降低(P<0.01);与模型组比较,越婢汤各剂量组细胞活力明显增加,细胞通透性明显降低,ASK1、p-p38蛋白表达明显降低,ZO-1、VE-cadherin蛋白表达明显升高(P<0.05,P<0.01)。结论越婢汤可改善阿霉素诱导的HUVEC高通透性,其作用机制可能与抑制ASK1/p38信号通路、上调细胞连接蛋白表达相关。Objective To investigate the effects of Yuebi Decoction on adriamycin-induced hyperpermeability of human umbilical vein vascular endothelial cells(HUVEC);To explore the possible mechanism of its therapeutic effect on edema in nephrotic syndrome.Methods HUVEC were divided into control group,model group and Yuebi Decoction low-,medium-,and high-dosage groups(200,300,400μg/mL).CCK-8 method was used to detect cell viability,Transwell method was used to detect cell permeability,Western blot was used to detect the expression of apoptosis signal regulated kinase 1(ASK1),zonula occludens-1(ZO-1),vascular endothelial-cadherin(VE-cadherin),and p38 protein,immunofluorescence method was used to detect the expression of ZO-1 and VE-cadherin.Results Compared with the control group,the cell activity of HUVEC in the model group was significantly decreased,cell permeability significantly increased,and cell swelling and membrane structure damage occurred,the expressions of ASK1 and p-p38 protein significantly increased,while the expressions of ZO-1 and VE-cadherin protein were significantly decreased(P<0.01).Compared with the model group,the cell activity and permeability of Yuebi Decoction low-,medium-,and high-dosage groups significantly increased,and the expressions of ASK1 and p-p38 protein were significantly decreased,and the expressions of ZO-1 and VE-cadherin protein significantly increased(P<0.05,P<0.01).Conclusion Yuebi Decoction can improve the high permeability of HUVEC induced by adriamycin,and its mechanism may be related to the inhibition of ASK1/p38 signaling pathway and upregulation of cell junction protein expression.

关 键 词：越婢汤 人脐静脉血管内皮细胞 肾病综合征 凋亡信号调节激酶1 P38 

分 类 号：R285.5[医药卫生—中药学]