基于Nrf2/HO-1信号通路探讨穴位埋线抑制脾肾阳虚夹瘀型溃疡性结肠炎大鼠氧化应激损伤作用机制  

Discussion on Mechanism of Acupoint Catgut Embedding in Inhibiting Oxidative Stress Injury in Rats with Ulcerative Colitis of Spleen-kidney Yang Deficiency and Blood Stasis Based on Nrf2/HO-1 Signaling Pathway

作　　者：谢超群 孙豪娴 张伟 邹孟龙 朱莹 汤美艳 XIE Chaoqun;SUN Haoxian;ZHANG Wei;ZOU Mengong;ZHU Ying;TANG Meiyan(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《中国中医药信息杂志》2024年第5期105-111,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81874466);湖南省中医药科研计划(D2022025);湖南省自然科学基金面上项目(2021JJ30531);湖南中医药大学研究生创新课题(2022CX34)。

摘　　要：目的观察穴位埋线对脾肾阳虚夹瘀型溃疡性结肠炎(UC)模型大鼠Nrf2/HO-1信号通路相关蛋白表达的影响,探讨穴位埋线治疗UC的可能机制。方法SPF级SD雄性大鼠随机分为空白组、模型组、柳氮磺吡啶(SASP)组和穴位埋线组,每组10只。除空白组外,其余各组通过腺嘌呤、番泻叶分阶段灌胃联合冰水浴+2,4,6-三硝基苯磺酸与乙醇混合试剂灌肠建立脾肾阳虚夹瘀型UC大鼠模型。穴位埋线组选取双侧“足三里”“天枢”“肾俞”“脾俞”“大肠俞”“膈俞”穴位埋线治疗,7d/次,共2次,SASP组予柳氮磺吡啶混悬液灌胃,空白组和模型组予生理盐水灌胃,1次/d,连续14d。称量大鼠体质量、观察大便性状和便血情况;测量大鼠结肠长度,观察结肠黏膜损伤情况,对结肠黏膜损伤指数(CMDI)进行评分;HE染色观察结肠组织病理形态变化;生化法测定大鼠血清丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性;RT-PCR和Westernblot分别检测结肠组织Kelch样ECH关联蛋白1(Keap1)、核因子E2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)mRNA和蛋白表达。结果与空白组比较,模型组大鼠体质量下降(P<0.01),结肠长度缩短(P<0.01),结肠黏膜形成明显溃疡面,隐窝结构丧失,腺体排列紊乱,大量炎性细胞浸润,CMDI评分升高(P<0.01),血清MDA含量增加,SOD、CAT、GSH-Px活性降低(P<0.01),结肠组织Keap1 mRNA和蛋白表达升高,Nrf2、HO-1 mRNA和蛋白表达降低(P<0.01);与模型组比较,SASP组和穴位埋线组大鼠体质量增加(P<0.01),结肠长度增加(P<0.01),结肠黏膜病理损伤明显减轻,CMDI评分降低(P<0.01),血清MDA含量降低,SOD、CAT、GSH-Px活性升高(P<0.01),结肠组织Keap1mRNA和蛋白表达降低,Nrf2、HO-1mRNA和蛋白表达升高(P<0.01)。SASP组和穴位埋线组各指标差异无统计学意义(P>0.05)。结论穴位埋线可减轻UC大鼠结肠黏膜病理损伤,其机制可能�Objective To observe the effects of acupoint catgut embedding on the expression of Nrf2/HO-1 signaling pathway related proteins in ulcerative colitis(UC)model rats with spleen-kidney yang deficiency and blood stasis;To explore the possible mechanism of acupoint catgut embedding in the treatment of UC.Methods SPF SD male rats were randomly divided into blank group,model group,SASP group and acupoint catgut embedding group,with 10 rats in each group.Except for the blank group,the other groups were established by adenine,senna phased gavage combined with ice water bath intervention+2,4,6-trinitrobenzene sulfonic acid and ethanol complex enema to establish a UC rat model with spleen-kidney yang deficiency and blood stasis.Acupoint catgut embedding group selected“Zusanli”,“Tianshu”,“Shenshu”,“Pishu”,“Dachangshu”and“Geshu”for acupoint catgut embedding treatment,7 days/time,twice.The SASP group was given SASP solution by gavage,and the blank group and the model group were given normal saline by gavage daily,once a day,for consecutive 14 d.The body mass,stool characteristics and hematochezia of rats in each group were observed;the colon length of rats was measured;the colonic mucosal injury was observed,and the score of colon mucosa damage index(CMDI)was evaluated;HE staining was used to observe the pathological change in colonic tissue;MDA content and SOD,CAT,and GSH-Px activities in serum were detected by biochemical method;RT-PCR and Western blot were used to detect the expressions of Keap1,Nrf2 and HO-1 mRNA and protein expression in colonic tissue.Results Compared with the blank group,the body mass of the rats in the model group significantly decreased(P<0.01),the colon length was significantly shortened(P<0.01),the colonic mucosa formed obvious ulcer surface,the crypt structure was lost,the gland arrangement was disordered,and a large number of inflammatory cells infiltrated,the CMDI score significantly increased(P<0.01),the content of MDA in serum increased,the activities of SOD,CAT and GSH

关 键 词：穴位埋线 溃疡性结肠炎 Nrf2/HO-1信号通路 氧化应激反应 

分 类 号：R245.9[医药卫生—针灸推拿学]