从网络药理学分析马齿苋-地锦草药对配伍治疗溃疡性结肠炎作用机制  

Analysis of the Action Mechanism of Portulacae-Quinquefolia Pairs in the Treatment of Ulcerative Colitis by Network Pharmacology

作　　者：李晶晶 王睿[2] 查安生[2] 李一芳 王芳[1] 胡子朝 LI Jing-jing;WANG Rui;CHA An-sheng;LI Yi-fang;WANG Fang;HU Zi-zhao(Anhui University of Traditional Chinese Medicine,Hefei 230038,China;The First Affiliated Hospital of Anhui University of Tradition Chinese Medicine,Hefei 230032,China;School of Nursing,Anhui University of Traditional Chinese Medicine,Hefei 230022,China)

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]安徽中医药大学第一附属医院,安徽合肥230032 [3]安徽中医药大学护理学院,安徽合肥230022

出　　处：《云南中医中药杂志》2024年第4期58-65,共8页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：安徽省教育厅高校自然科学研究重点项目(KJ2020A0445)。

摘　　要：目的运用网络药理学技术探讨马齿苋-地锦草药对治疗溃疡性结肠炎(UC)作用机制。方法通过TCMSP数据库获取马齿苋-地锦草的有效活性成分,利用Uniprot3.7.2数据库预测活性成分作用靶点,通过Gene-Cards数据库、OMIM数据库获取疾病基因,将药物与疾病基因取交集后利用STRING数据库研究二者相关性,并根据结果分析其潜在作用机制。结果经过筛选得到目标药对关键靶点蛋白61个和分子信号通路169条,基因本体(GO)功能富集分析显示主要有炎症反应、凋亡过程的负调控、脂多糖的细胞反应等生物过程;京都基因和基因组百科全书(KEGG)富集分析显示马齿苋-地锦草治疗UC主要通过肿瘤坏死因子(TNF)信号通路、核因子κB信号通路、Toll样受体信号通路、NOD样受体信号通路等发挥作用。结论马齿苋-地锦草药对治疗UC具有多靶点、通路复杂、层次分明的特点,马齿苋-地锦草潜在作用机制为日后临床治疗UC提供理论依据,并拓展了临床运用中医药治疗UC的思路。Objective:To explore the action mechanism of Portulaca-Quinquefolia pairs in the treatment of ulcerative colitis(UC)by network pharmacology.Methods:The active ingredients of Portulaca and Quinquefolia were obtained through TCMSP database,the target of active ingredients was predicted by using Uniprot3.7.2 database,and disease genes were obtained through Gene-Cards database and OMIM database.After the intersection of herbs and disease genes,the correlation between them was studied by using STRING database.The potential mechanism of action was analyzed according to the results.Results:After screening,61 key target proteins and 169 molecular signaling pathways were obtained.Gene ontology(GO)functional enrichment analysis showed that the main biological processes were inflammation,negative regulation of apoptosis and cell reaction of lipopolysaccharide.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that Portulaca-Quinquefolia pairs played its role in the UC treatment mainly through tumor necrosis factor(TNF)signaling pathway,nuclear factorκB signaling pathway,toll-like receptor signaling pathway and NOD-like receptor signaling pathway.Conclusion:Portulaca-Quinquefolia pairs have the characteristics of multiple targets,complex pathways and distinct layers in the treatment of UC patients.The potential action mechanism of Portulaca-Quinquefolia pairs provides a theoretical basis for the future clinical treatment of UC,and expands the clinical application of TCM in the treatment of UC.

关 键 词：马齿苋-地锦草药对 网络药理学 溃疡性结肠炎 信号通路 作用机制 

分 类 号：R516.1[医药卫生—内科学]