乌头属植物中生物碱毒素检验方法及溯源研究进展  

Research progress on detection methods and traceability of alkaloid toxins in Aconitum species

作　　者：刘天宇[1] 宋歌 杨瑞琴[1] 张云峰 张成龙 LIU Tian-yu;SONG Ge;YANG Rui-qin;ZHANG Yun-feng;ZHANG Cheng-long(School of Investigation,People's Public Security University of China,Beijing 100038,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]中国人民公安大学侦查学院,北京100038 [2]公安部鉴定中心,北京100038

出　　处：《药学学报》2024年第4期899-907,共9页Acta Pharmaceutica Sinica

基　　金：中国人民公安大学刑事科学技术双一流创新研究专项(2023SYL06);中央级公益性科研院所基本科研业务费库重点项目(2022JB004).

摘　　要：作为乌头属植物中最主要的化学毒素成分,乌头类生物碱(Aconitum alkaloids,ATs)同时具有重要的药用价值,在传统中医药以至现代临床医学领域都有极为广泛的应用。然而,受其剧毒毒性的影响,ATs在药品、食品、环境中的不合理使用会对人体健康造成严重威胁,由此引发一系列中毒案件。因此,开发有效的检验方法至关重要。文中综述了常见基质中ATs前处理和检验方法的研究进展,包括高效液相色谱法、液相色谱-质谱联用法以及快速检测方法。此外,为进一步探究实际中毒案例中ATs的具体来源,探讨了基于植物形态学、化学指纹图谱分析以及DNA条形码技术的综合溯源策略,对ATs的检验和溯源技术发展提出综合性的展望,以期为法庭科学领域相关研究提供参考。As the predominant toxic constituent within the Aconitum genus,Aconitum alkaloids(ATs)exhibit both significant pharmaceutical value and substantial toxicity,have been widely used in traditional Chinese medicine and the realm of contemporary clinical medicine.However,owing to their high toxicity,inappropriate employment of ATs in pharmaceuticals,edibles,and the environment will pose serious threats to human health,inciting a series of toxic incidents.Consequently,it is very important to develop effective analytical methods.This paper presents a comprehensive review of the advancements in research pertaining to the pretreatment and detection methods in common substrates,including high performance liquid chromatography,liquid chromatography-mass spectrometry and rapid detection methods.To explore the specific sources of ATs in actual poisoning cases,the comprehensive traceability strategy based on plant morphology,chemical fingerprint analysis and DNA barcoding technology was discussed,proposing a comprehensive prospect for the development of ATs analysis and traceability,in order to provide guidance for related research within the forensic science domain.

关 键 词：乌头属植物 生物碱 检测 指纹图谱 溯源 

分 类 号：D918.9[政治法律—法学] O657.7[理学—分析化学]