白花败酱中三萜类化学成分研究  

Study on the chemical constituents of triterpenoids in Patrinia villosa Juss.

作　　者：吴其国[1,3] 黄乐怡 陈志 刘东风[5] 王永丽 侴桂新 WU Qi-guo;HUANG Le-yi;CHEN Zhi;LIU Dong-feng;WANG Yong-li;CHOU Gui-xin(Department of Pharmacy,Anqing Medical College,Anqing 246052,China;Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai R&D Centre for Standardization of Chinese Medicines,Shanghai 201203,China;State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry Co.,Ltd.,China State Institute of Pharmaceutical Industry,Shanghai 201203,China;Anqing food and drug inspection Center,Anqing 246000,China)

机构地区：[1]安庆医药高等专科学校药学院,安徽安庆246052 [2]上海中医药大学中药研究所,上海201203 [3]上海中药标准化研究中心,上海201203 [4]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海201203 [5]安庆食品药品检验中心,安徽安庆246000

出　　处：《药学学报》2024年第4期1005-1009,共5页Acta Pharmaceutica Sinica

基　　金：“十三五”科技重大专项“中药组分资源库”子课题-中药组分及对照品制备(2019ZX09735002-004);安徽省教育厅高校科学研究重大项目(2022AH040323);安庆医药高等专科学校科创团队项目(KCTD202304)。

摘　　要：采用硅胶柱色谱、中压液相色谱、葡聚糖凝胶Sephadex LH-20柱色谱及制备液相色谱等多种色谱技术,从白花败酱95%乙醇提取物中分离得到7个三萜类化合物。根据化合物的理化性质和波谱数据确定了它们的结构,分别为29-乙酰氧基齐墩果酸-3-O-α-L-阿拉伯糖苷(1)、齐墩果酸(2)、3β-羟基-24-降-乌苏-4(23),12(13),20(30)-三烯-28-酸(3)、3β-羟基-24-降-乌苏-4(23),12-二烯-28-酸(4)、熊果酸(5)、常春藤皂苷元(6)、齐墩果酸3-O-阿拉伯糖苷(7)。化合物1为新化合物,化合物3、4、6、7为首次从该植物中分离得到。通过对脂多糖(LPS)诱导的RAW 264.7细胞(小鼠单核巨噬细胞)释放的一氧化氮(NO)影响实验测定了化合物的抗炎活性,结果表明化合物1、2、3、4、6、7有显著抑制NO作用。Seven triterpenoids were isolated and purified from the 95%aqueous EtOH extract whole plants of P.villosa by various chromatographic techniques,such as silica gel,ODS,Sephadex LH-20 gel column chromatography and preparative high performance liquid chromatography.Based on physicochemical properties and spectral analyses,the structures of the seven compounds were identified as 29-acetoxyoleanolic acid-3-O-α-L-arabinopyranoside(1),oleanolic acid(2),3β-hydroxy-24-norursa-4(23),12,20(30)-trien-28-oic acid(3),3β-hydroxy-24-nor-urs-4(23),12-dien-28-oic acid(4),ursolic acid(5),hederagenin(6),oleanolic acid 3-O-arabinoside(7).Compound 1 is a new compound.Compounds 3,4,6,and 7 are isolated from P.villosa for the first time.All compounds were assayed for their anti-inflammatory activity by the prodution of NO in lipopolysaccharide-stimulated RAW 264.7 cells.The results showed that compounds 1,2,3,4,6,and 7 significantly inhibited the NO release.

关 键 词：白花败酱 三萜 抗炎 化学成分 

分 类 号：R284[医药卫生—中药学]