ICP-MS法结合化学计量学分析不同批次地龙多肽中无机元素的含量  

Analysis of inorganic elements in different batches of earthworm polypeptides by ICP-MS combined with chemometrics technology

作　　者：杨洪柳 仲伟婷 郭雨师 李姝琪 亓金钗 刘永刚[1] 马涛 YANG Hong-liu;ZHONG Wei-ting;GUO Yu-shi;LI Shu-qi;QI Jin-chai;LIU Yong-gang;MA Tao(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102401,China;Beijing Tide Pharmaceutical Co.,Ltd.,Beijing 100176,China;Baotou Kundulun District Hospital,Baotou 014010,China)

机构地区：[1]北京中医药大学中药学院,北京102401 [2]北京泰德制药股份有限公司,北京100176 [3]包头市昆都仑区医院,内蒙古包头014010

出　　处：《药学学报》2024年第4期1040-1047,共8页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划(2018YFC1706505).

摘　　要：为建立测定地龙多肽中26种无机元素的方法,测定不同批次地龙多肽中的元素含量,本研究采用微波消解法对样品进行前处理,以ICP-MS法对不同批次地龙多肽中26种元素进行含量测定。26种元素在0~1000μg·L^(-1)内线性关系良好,R2大于0.999,精密度RSD为0.21%~2.71%,重复性RSD为0.19%~4.69%,稳定性RSD为0.11%~4.24%,加样回收率为82.41%~116.16%。数据采用Origin 2022软件绘制元素含量分布特征图,利用SPSS 27.0进行主成分分析,采用SIMCA 14.1软件进行OPLS-DA分析。结果表明,26种元素中,地龙多肽含量较高的常量元素是K、Na和Ca,其次主要含有Zn、Fe、Al、B等微量元素,In、Sc、Co、Pb和Bi等元素含量极少甚至未能检出,且不同批次地龙多肽元素含量存在差异。本研究通过对地龙多肽元素种类和含量进行测定分析,为地龙多肽的生产质量控制及质量评价、药效应用提供一定的理论基础和依据。To establish a method for determining 26 inorganic elements in earthworm polypeptide and determine the elemental content in different batches of earthworm polypeptide,microwave digestion method was used to pre-treat the samples,and ICP-MS method was used to determine the content of 26 elements in different batches of earthworm polypeptide.The linear relationships of 26 elements were good in the range of 0-1000μg·L^(-1),with R2 greater than 0.999,precision RSD 0.21%-2.71%,repeatability RSD 0.19%-4.69%,stability RSD 0.11%-4.24%,and recovery rates of 82.41%-116.16%.The data was plotted using Origin 2022 software to characterize the distribution of elements content.SPSS 27.0 was used for principal component analysis,and SIMCA 14.1 software was used for OPLS-DA analysis.The results showed that among the 26 elements,the higher content of earthworm polypeptide was K,Na,and Ca,followed by Zn,Fe,Al,B and other trace elements,In,Sc,Co,Pb,Bi and other elements had little or no detectable content,and there were differences in the content of polypeptide in different batches.This study provides a theoretical basis for the production quality control,quality evaluation and drug efficacy application of earthworm polypeptide through the determination and analysis of the elements and content of earthworm polypeptide.

关 键 词：电感耦合等离子体-质谱法 微波消解 地龙 地龙多肽 无机元素 

分 类 号：R917[医药卫生—药物分析学]