限根栽培对4个宁夏枸杞品种土壤养分和微生物区系的影响  

Effects of root-limited cultivation on soil nutrients and microbial flora of four Lycium barbarum cultivars

作　　者：何军 田英 何昕孺 朱丽珍 王振平[3] 李晓莺 HE Jun;TIAN Ying;HE Xinru;ZHU Lizhen;WANG Zhenping;LI Xiaoying(The Wolfberry Sciences Research Institute,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,Ningxia,China;Institute of Forestry and Grassland Ecology,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,Ningxia,China;School of Agriculture,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏农林科学院枸杞科学研究所,宁夏银川750002 [2]宁夏农林科学院林业与草地生态研究所,宁夏银川750002 [3]宁夏大学农学院,宁夏银川750021

出　　处：《经济林研究》2024年第1期150-158,共9页Non-wood Forest Research

基　　金：国家重点研发计划项目(2020YFD10007);宁夏重点研发计划项目(2021BEF02004);宁夏农林科学院农业高质量发展和生态保护科技创新示范项目(NGSB-2021-2);宁夏人才专项。

摘　　要：【目的】为宁夏中部干旱地区宁夏枸杞限根栽培提供参考,促进枸杞生态种植。【方法】在宁夏中部干旱带同心县王团镇典型土壤气候类型区,选择同一限根栽培条件下种植3 a的4个宁夏枸杞品种(宁杞1号、宁杞7号、科杞6081、科杞6082)为研究对象,探讨限根栽培模式下不同宁夏枸杞品种之间根域土壤理化性质的差异,并利用Illumina HiSeq高通量技术和冗余分析方法研究根域土壤细菌、真菌的群落结构和多样性,分析土壤理化性质和微生物群落特征之间的关系。【结果】除科杞6082的土壤全氮含量略高于科杞6081外,科杞6081的根域土壤含水量、全氮含量、全磷含量、全钾含量均为最高,较宁杞1号土壤分别显著高出27.41%、37.41%、17.96%、2.31%,较宁杞7号土壤分别显著高出17.42%、24.69%、15.60%、4.53%,土壤含水量、全磷含量、全钾含量较科杞6082土壤分别显著高出16.93%、10.73%、3.49%;科杞6082根域土壤的有机碳含量最高,较宁杞1号、宁杞7号和科杞6081根域土壤分别显著高出26.73%、5.82%和14.20%;宁杞1号和科杞6081根域土壤的pH值显著高于宁杞7号和科杞6082。限根栽培下4个宁夏枸杞品种土壤优势细菌门基本相同,其中,变形菌门Proteobacteria所占比例较大,4个宁夏枸杞品种丰度均在35%以上;真菌属群落组成差异较大,特别是科杞6081根域土壤的真菌群落表现出一定的差异性;在RDA分析结果中科杞6081根域土壤的真菌门水平优势群落富集在表征土壤养分的指标周围,其他3个宁夏枸杞品种相对较为分散。土壤全氮含量、有机碳含量、全磷含量等与土壤细菌、真菌群落组成和丰度存在正相关关系。【结论】在限根栽培条件下,4个宁夏枸杞品种根域土壤的主要养分含量存在差异,对细菌群落结构组成和多样性无影响,但真菌差异较大,科杞6081的土壤理化性质和真菌群落组成均表现出相对较大的差异【Objective】In order to provide a reference for root-limited cultivation of Lycium barbarum in the arid area of central Ningxia and promote ecological planting of L.barbarum.【Method】Taking four L.barbarum(four cultivars:Ningqi No.1,Ningqi No.7,Keqi 6081,Keqi 6082)that were cultivated for 3 years under the same root-limited cultivation conditions were selected as the research objects to explore the differences of soil nutrients in root zone among different cultivars under the root-limited cultivation mode,in the typical soil climate type area of Wangtuan town,Tongxin county,the central arid zone of Ningxia,and used Illumina HiSeq high-throughput technology and redundancy analysis method to study the community structure and diversity of soil bacteria and fungi in root zone,to analysis the relationships between soil nutrients and microbial community characteristics.【Result】Water content(WC),total nitrogen(TN),total phosphorus(TP)and total potassium(TK)contents of root zone soil of Keqi 6081 were the highest(except that the soil total nitrogen content of Keqi 6082 was slightly higher than that of Keqi 6081),which were 27.41%,37.41%,17.96%,2.31%higher than those of Ningqi No.1 soil,17.42%,24.69%,15.60%,and 4.53%higher than those of the Ningqi No.7 soil,and 16.93%,10.73%,3.49%higher than those of the Keqi 6082 soil,respectively;the soil organic carbon(SOC)content of the Keqi 6082 was the highest,which was significantly higher than that of the soil of Ningqi No.1,Ningqi No.7 and Keqi 6081 by 26.73%,5.82%and 14.20%,respectively;pH of soil of Ningqi No.1 and Keqi 6081 were significantly higher than that of Ningqi No.7 and Keqi 6082(P<0.05).The soil dominant bacterial phyla of the four L.barbarum cultivars under root-limited cultivation were basically the same.Among them,proteobacteria accounted for a larger proportion at the phylum level,and the abundance of the four cultivars soil was 35%or more,while the community composition of fungal genera was quite different,especially the soil fungi in the root zone of t

关 键 词：限根栽培 宁夏枸杞品种 土壤理化性质 细菌 真菌 微生物区系 根域 

分 类 号：S663.9[农业科学—果树学]