一种检测生物硫醇和硫化物的NBD荧光探针  

ANBD-based Fluorescent Probe for the Detection of Biothiols and Sulfides

作　　者：杨静 王志宇 张雪 胡磊 王慧 YANG Jing;WANG Zhiyu;ZHANG Xue;HU Lei;WANG Hui(College of Pharmacy,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《咸阳师范学院学报》2024年第2期31-35,114,共6页Journal of Xianyang Normal University

基　　金：安徽省自然科学基金项目(1908085QB50);安徽省高校自然科学研究项目(2023AH051762,2023AH051747);安徽省大学生创新创业教育训练计划项目(S200310368089)。

摘　　要：文中合成了一种新型的荧光探针LD-TPA-NBD,系统研究了探针响应生物硫醇和硫化物的性质。结果表明,探针LD-TPA-NBD在380 nm和475 nm的激发波长下,可以特异性的识别不同的活性硫物质,对Cys、GSH、Hcy、HS-和HSO3-的检测限分别为47.7 nM、72.2 nM、76.9 nM、0.34μM和0.207μM(λ_(ex)=380 nm)。此外,通过研究探针LD-TPA-NBD的荧光动力学,发现探针的荧光信号在3~7 min内即可达到平衡。探针LD-TPA-NBD可为用于检测活性硫物质的荧光探针的构建提供参考。A novel fluorescent probe LD-TPA-NBD was synthesized and the properties of the probe in response to biothiols and sulfides were systematically investigated.The results indicated that the probe LD-TPA-NBD can specifically identify different active sulfur species at the excitation wavelengths of 380 nm and 475 nm,respectively.The detection limits of the probe for Cys,GSH,Hcy,HSand HSO3-were 47.7 nM,72.2 nM,76.9 nM,0.34μM and 0.207μM(λ_(ex)=380 nm),respectively.In addition,by studying the fluorescence dynamics of the probe LD-TPA-NBD,it was found that the fluorescence signal of this probe can level off within 3 min-7 min.Probe LD-TPA-NBD can provide a reference for the discovery of fluorescent probes for the detection of active sulfur species.

关 键 词：荧光探针 生物硫醇 硫化氢 亚硫酸氢钠 荧光光谱 

分 类 号：O625.6[理学—有机化学]