AIFM1分子与猪伪狂犬病病毒UL16蛋白互作抑制病毒的增殖机制研究  

Research for the Mechanism of Inhibition of Viral Propagation Through AIFM1 Interaction with Pseudorabies Virus UL16 Protein

作　　者：徐晶晶[1] 高飞[1,2] 武吉强 程雪飞 刘宇婷 傅欣玲 郑浩 童武[1,2] 童光志 李国新[1,2] XU Jingjing;GAO Fei;WU Jiqiang;CHENG Xuefei;LIU Yuting;FU Xinling;ZHENG Hao;TONG Wu;TONG Guangzhi;LI Guoxin(Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]扬州大学江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国动物传染病学报》2024年第1期9-17,共9页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划项目(2016YFD0500100);广东省重点领域研发计划项目(2019B020211003)。

摘　　要：凋亡诱导因子1(AIFM1)是一个结构复杂的线粒体蛋白,能优化线粒体呼吸链的功能、参与氧化还原代谢,是第一个被发现的caspase非依赖性的凋亡蛋白。本研究发现AIFM1能与猪伪狂犬病病毒(PRV)pUL16相互作用,过表达以及RNA干扰试验结果表明,AIFM1能抑制PRV的增殖,且pUL16能减弱这种抑制作用。激光共聚焦和流式试验表明,PRV感染细胞时,AIFM1从线粒体易位至细胞核,且AIFM1增强了PRV引起的细胞凋亡。本研究通过对PRV pUL16和宿主蛋白相互作用机制研究,部分地解析了这两种蛋白在PRV复制、包装以及与宿主互作中发挥的作用,为进一步阐明PRV感染机制奠定了基础。Apoptosis-inducing factor 1(AIFM1)is a complex mitochondrial protein,which can optimize the function of mitochondrial respiratory chain and participate in redox metabolism.It is thefirst caspase-independent apoptotic protein discovered so far.In this study,AIFM1 was found to interact with pseudorabies virus(PRV)pUL16.The results of overexpression and RNA interference showed that AIFM1 inhibited the proliferation of PRV and pUL16 weakened this inhibition.The laser confocal andflow test showed that AIFM1 translocated from mitochondria to nuclei when PRV infected cells and AIFM1 enhanced apoptosis induced by PRV.The interaction mechanism between PRV pUL16 and host protein AIFM1 was investigated and the roles of these two proteins in viral replication,packaging and interaction with host were partially analyzed in the present study,which laid a foundation for further elucidating PRV infection mechanism.

关 键 词：伪狂犬病病毒 独特长区16蛋白 凋亡诱导因子1 

分 类 号：S852.651[农业科学—基础兽医学]