牛病毒性腹泻病毒1型KE-9基因缺失弱毒疫苗和牛病毒性腹泻病毒2型NY-93基因缺失弱毒疫苗在怀孕母牛中垂直传播能力评价  

Assessment of the Vertical Transmission Ability of BVDV Type 1 KE-9 Gene Deleted Live Vaccine and BVDV Type 2 NY-93 Gene Deleted Live Vaccine in Pregnant Heifers

作　　者：孙艳永 田赵勇 徐瑞 陈宁 文世富 SUN Yanyong;TIAN Zhaoyong;XU Rui;CHEN Ning;WEN Shifu(Boehringer-Ingelheim Vetmedica(China)Co.,Ltd.,Taizhou 225300,China)

机构地区：[1]勃林格殷格翰动物保健(中国)有限公司,泰州225300

出　　处：《中国动物传染病学报》2024年第1期82-87,共6页Chinese Journal of Animal Infectious Diseases

摘　　要：本研究通过对怀孕母牛免疫牛病毒性腹泻病毒1型KE-9基因缺失弱毒疫苗和牛病毒性腹泻病毒2型NY-93基因缺失弱毒疫苗后检测胎牛组织中是否存在疫苗病毒,以评价该疫苗的垂直传播能力。试验将16头牛病毒性腹泻病毒(BVDV)抗原和抗体阴性,怀孕60~90 d的母牛随机分成2组,第1组6母牛肌肉注射2.0 mL牛病毒性腹泻病毒1型KE-9基因缺失弱毒疫苗;另外2头牛不做任何接种作为哨兵牛同群饲养。第2组6母牛肌肉注射2.0 mL牛病毒性腹泻病毒2型NY-93基因缺失弱毒疫苗;另外2头牛不做任何接种作为哨兵牛同群饲养。母牛免疫后血清抗体转阳,BVDV抗体明显升高。免疫后第56 d剖取免疫牛的胎牛,采集胎牛脑、胸腺、肠系膜淋巴结和脾脏组织用病毒分离方法检测牛病毒性腹泻病毒。结果显示,所有怀孕母牛在整个试验期内无任何临床症状,也无流产现象。试验结束时胎牛发育良好,且未在胎牛的脑、胸腺、肠系膜淋巴结和脾脏组织中分离到牛病毒性腹泻病毒。综上所述,牛病毒性腹泻病毒1型KE-9基因缺失弱毒疫苗和牛病毒性腹泻病毒2型NY-93基因缺失弱毒疫苗对怀孕母牛和胎牛都是安全的,无垂直传播。The study aimed to assess the vertical transmission ability of BVDV type 1KE-9gene deleted live vaccine and BVDV type 2 NY-93 gene deleted live vaccine in pregnant heifers.Sixteen 60-90 days of gestation heifers were randomly assigned to two groups with eight heifers in each group.Six heifers in Group 1 were vaccinated intramuscularly with 2.0 mL BVDV type 1 KE-9 gene deleted live vaccine,and another two heifers in Group 1 were served as sentinel animals without any treatment.Six heifers in Group 2 were vaccinated with 2.0 mL BVDV type 2NY-93gene deleted live vaccine,and another two heifers in Group 2 were served as sentinel animals without any treatment.Vaccination induced seroconversion was found on Day 56 for all vaccinated heifers.The foetuses were collected from all vaccinated cows at 56 days after vaccination by caesarean section.The foetuses’brain,thymus,mesenteric lymph nodes and spleen were collected for BVDV virus isolation.The study results showed that no abortion was found in the present study for all vaccinated heifers.Neither BVDV type 1KE-9gene deleted live vaccine,nor BVDV type 2NY-93gene deleted live vaccine had been isolated in any of the analysed tissues from the foetuses on 56 days after vaccination.Consequently,both BVDV type 1 KE-9 gene deleted live vaccine and BVDV type 2NY-93gene deleted live vaccine are safe in pregnant heifers and their foetuses with regard to vertical transmission.

关 键 词：牛病毒性腹泻病毒 基因缺失弱毒疫苗 垂直传播 

分 类 号：S858.23[农业科学—临床兽医学]