34株鸭疫里默氏杆菌的分离鉴定及部分耐药基因调查分析  

Isolation,Identifi cation and Drug Resistance Genes of 34 Riemerella anatipestifer Isolates

作　　者：张燕 董洪燕 于圣青[3] 杨静静 吴植[2] 吴双[2] 洪双鹏 朱善元[2] 邢华[1] ZHANG Yan;DONG Hongyan;YU Shengqing;YANG Jingjing;WU Zhi;WU Shuang;HONG Shuangpeng;ZHU Shanyuan;XING Hua(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Jiangsu Guiliu Animal Husbandry Group Corporation,Xuzhou 221000,China)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]江苏农牧科技职业学院,泰州225300 [3]中国农业科学院上海兽医研究所,上海200241 [4]江苏桂柳牧业集团有限公司,徐州221000

出　　处：《中国动物传染病学报》2024年第1期142-149,共8页Chinese Journal of Animal Infectious Diseases

基　　金：江苏省农业科技自主创新资金项目(CX(18)1004);泰州市科技支撑计划(农业)项目(TN201801);江苏现代农业产业技术体系集成创新中心(JATS[2020]345);江苏省高等学校自然科学研究重大项目(20KJA230004);江苏高校“青蓝工程”项目(苏教师函(2020)10号)。

摘　　要：为更好的指导养殖场鸭疫里默氏杆菌病临床用药以及探究该菌多重耐药机制,本研究对安徽地区患病鸭采样、细菌分离鉴定后,进行生化鉴定、药敏试验,再根据药敏结果进行氨基糖苷类耐药基因以及Ⅰ类整合子鉴定。药敏试验结果表明该地区34株鸭疫里默氏杆菌分离株对头孢拉定、头孢曲松、氟苯尼考、大观霉素、磺胺异恶唑等抗生素较敏感,对卡那霉素、庆大霉素、丁胺卡那、链霉素、阿奇霉素等抗生素均有较强的耐药性;其中有97%的菌株存在多重耐药现象,最高可耐19种抗生素。15种氨基糖苷类耐药基因鉴定结果表明aac(6')Ⅰb基因、aac(6')Ⅱ基因、rmtC基因、aph(3')Ⅱa基因检出率分别为94.1%(32/34)、82.4%(28/34)、73.5%(25/34)、70.6%(24/34),其余基因均未检测到;同时34株分离株均携带Ⅰ类整合子,大小为1 kb,其基因盒种类为aadA1(链霉素耐药基因);其氨基糖苷类耐药表型和耐药基因符合率大于69%。因此,安徽地区鸭疫里默氏杆菌分离株多重耐药性严重,需加强对抗生素的用药规范性,特别要注意氨基糖苷类药物使用。To better guide the clinical medication of farms and explore the mechanism of multi-drug resistance about Riemerella anatipestifer,bacteria were isolated from moribund ducks in Anhui province in this study.Total 34 RA isolates were obtained by biochemical reaction and tested for drug susceptibility.Then aminoglycoside resistance genes and class I integrons were identified according to the results of drug susceptibility.Drug susceptibility test showed that these 34 isolates were more sensitive to cefradine,ceftriaxone,florfenicol,spectinomycin and sulfisoxazole but strong resistant to kanamycin,gentamicin,amikacin,streptomycin and azithromycin.In addition,97%RA isolates were multi-drug resistant and the highest resistance reached up to 19 antibiotics.Examination of 15 aminoglycoside resistance genesrevealed varying detection rates of aac(6')Ib gene(94.1%,32/34),aac(6')IIgene(82.4%,28/34),rmtC gene(73.5%,25/34)and aph(3')IIa gene(70.6%,24/34).Other drug resistant genes were not detected.At the same time,all 34 isolates carried class I integrons,the size of which was 1 kb and the gene cassette type was aadA1(streptomycin resistance gene).The coincidence rate of drug resistance phenotype and drug resistance genes was greater than 69%.Therefore,the multi-drug resistance was severe among RA isolates in Anhui province indicating a necessity to strengthen the standardization of the antibiotic use and pay special attention to aminoglycoside antibiotics.

关 键 词：鸭疫里默氏杆菌病 药敏试验 耐药基因 Ⅰ型整合子 

分 类 号：S852.61[农业科学—基础兽医学]