Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite  

作　　者：Huan Liang Hongcheng Liu Haojian Lin Guobao Ning Xiaokang Lu Siying Ma Fei Liu Hui Zhao Canpeng Li 

机构地区：[1]School of Chemical Science and Technology,Yunnan University,Key Laboratory of Medicinal Chemistry for Natural Resource,Ministry of Education,Kunming 650091,China [2]Institute of Quality Standard and Testing Technology,Yunnan Academy of Agricultural Science,Supervision and Testing Center for Farm Product Quality,Ministry of Agriculture,Kunming 650223,China [3]State Key Laboratory of Optoelectronic Materials and Technologies,Guangdong Province Key Laboratory of Display Material and Technology,School of Electronics and Information Technology,Sun Yat-sen University,Guangzhou 510275,China [4]Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China

出　　处：《Food Science and Human Wellness》2024年第4期2025-2035,共11页食品科学与人类健康（英文）

基　　金：This work was financially supported by Major Science and Technology Project of Yunnan Province(202302AE090022);Key Research and Development Program of Yunnan(202203AC100010);the National Natural Science Foundation of China(32160597,32160236,32371463);National Key Research and Development Program of China(2022YFC2601604);Cardiovascular Ultrasound Innovation Team of Yunnan Province(202305AS350021);Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001);the second phase of“Double-First Class”Program Construction of Yunnan University.

摘　　要：Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.

关 键 词：Staphylococcus aureus enterotoxin Electrochemical immunosensor Colorimetric assay MOF@borophene composite Dual-functional Fe-N-C signal atom catalyst 

分 类 号：O657[理学—分析化学] TS207.5[理学—化学]