植物内生真菌Purpureocillium lilacinum的生物碱类次级代谢产物  

Alkaloid secondary metabolites from a plant endophytic fungus Purpureocillium lilacinum

作　　者：唐官美 张丽[1] 韦媛 付姣 陈惠琴[2] 张雪晴 梅文莉[2] 戴好富[2] 郭志勇[1] TANG Guanmei;ZHANG Li;WEI Yuan;FU Jiao;CHEN Huiqin;ZHANG Xueqing;MEI Wenli;DAI Haofu;GUO Zhiyong(Hubei Key Laboratory of Natural Product Research and Development(Three Gorges University),College of Biological and Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,Hubei,China;Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China)

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室生物与制药学院,湖北宜昌443002 [2]中国热带农业科学院热带生物技术研究所海南省黎药资源天然产物研究与利用重点实验室,海南海口571101

出　　处：《菌物学报》2024年第4期95-103,共9页Mycosystema

基　　金：海南省黎药资源天然产物研究与利用重点实验室开放基金(202204)。

摘　　要：从蛛毛苣苔叶片中分离一株内生真菌GZY-Z-02,经分子生物学方法鉴定为Purpureocillium lilacinum,以大米固体培养基于室温培养30d,经乙酸乙酯浸提,以正向硅胶、凝胶色谱及HPLC等手段分离得到12个化合物,通过核磁共振、质谱及CD谱等手段鉴定其结构,12个化合物均为生物碱类次级代谢产物,其中新化合物1个,为lilacinumopin(1),5个环二肽类衍生物(2–6),2个简单吲哚类衍生物(7–8),3个核苷类衍生物(9–11)及核黄素(12)。通过抗菌活性筛选,发现化合物1,7和8有中等强度地抑制金黄葡萄球菌的活性。An endophytic fungus strain GZY-Z-02 was isolated from the leaves of Paraboea sinensis and identified as Purpureocillium lilacinum by molecular biological method.The fungus was fermented on rice medium for 30 days at room temperature,and the crude extracts were obtained by extraction with ester acetate.By means of silica gel column chromatography,Sephadex LH-20 and HPLC twelve metabolites were obtained.The twelve metabolites were identified through NMR,MS and CD spectra,which all belong to alkaloids,including a new compound lilacinumopin(1),five diketopiperazines(2–6),two indole analogs(7–8),three nucleoside derivatives(9–11)and Vitamin B2(12).An antimicrobial activity assay proved that the compounds 1,7,and 8 displayed selectively moderate antibacterial activity to Staphylococcus aureus.

关 键 词：植物内生真菌 生物碱 抗菌活性 淡紫拟青霉 

分 类 号：R284[医药卫生—中药学]