基于BSR-Seq分析筛选Y1499条锈病抗性相关基因  

Screening of Stripe Rust Resistance Related Genes in Y1499 via BSR-Seq Analysis

作　　者：蒋雯婷 王光浩 郭欢 黄晨曦 张宏[1] 吉万全[1] JIANG Wenting;WANG Guanghao;GUO Huan;HUANG Chenxi;ZHANG Hong;JI Wanquan(College of Agronomy,Northwest A&F University/Yangling Sub-Centre,National Wheat Improvement Centre,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院/国家小麦改良中心杨陵分中心,陕西杨凌712100

出　　处：《麦类作物学报》2024年第5期577-584,共8页Journal of Triticeae Crops

基　　金：陕西省自然科学基金项目(2021JM-090)。

摘　　要：条锈病是危害小麦生产安全的重要病害之一,而携带不同抗病基因小麦品种的利用与合理布局是降低病害威胁最经济可靠的途径。为挖掘和丰富小麦条锈病抗性相关基因资源,本研究以筛选到的抗条锈病硬粒小麦种质Y1499和感病材料Gaza为亲本构建遗传群体,在室内对其进行苗期抗性鉴定和统计分析;利用衍生后代中选择的F 3∶4群体构建混池,进行RNA测序,对测序数据进行相关分析。结果表明,Y1499对CYR32的抗性由两个主效基因互补遗传控制。利用BSR-Seq关联分析在鉴定到3个小麦材料Y1499中条锈病抗性基因候选区间。GO富集结果显示,F 3∶4抗感池间差异表达基因涉及多个过氧化物酶等抗氧化反应相关基因。KEGG富集结果表明,显著差异表达基因主要富集于谷胱甘肽代谢和苯丙烷类生物合成等代谢通路。Wheat stripe rust is an important disease that threatens the safety of wheat production,while utilization and deployment of disease resistant varieties is the most economical way to control the disease.In order to explore and enrich the genes related to stripe rust resistance,this study constructed a genetic F 2 population with stripe rust resistance emmer germplasm Y1499 and susceptible material Gaza.Seedlings of the parents,F 1 and F 2 progeny were evaluated for resistance to race CYR32.Two F 3∶4 populations from their offsprings were selected to construct bulked pools for RNA sequencing,and then sequencing data were analyzed.The results showed that the resistance of Y1499 to CYR32 was controlled by two major genes through dominant complementary inheritance.Meanwhile,three candidate intervals related to stripe rust resistance genes in wheat material Y1499 were identified using BSR-Seq analysis.Comparing resistance with susceptible bulk of F 3∶4 populations,GO enrichment analysis showed that the differentially expressed genes(DEGs)involved multiple antioxidant-related genes such as peroxidase,while the results of KEGG enrichment analysis showed that DEGs were significantly enriched in the metabolic pathways of glutathione metabolism and phenylpropane biosynthesis.

关 键 词：硬粒小麦 条锈病 BSR-Seq 抗病相关基因 

分 类 号：S512.1[农业科学—作物学] S330