高效液相色谱法和气相色谱法分析肟菌酯原药的方法比较  

Comparison of methods for original drug of trifloxystrobin by HPLC and GC

作　　者：陈颖 刘小锋 陈一萍 廖丽萍 傅洪涛 Chen Ying;Liu Xiaofeng;Chen Yiping;Liao Liping;Fu Hongtao(National Engineering Research Center for Agriculturals,Hunan Research Institute of Chemical Industry Co.,Changsha,Hunan 410014;Supervision and Inspection Authorization Station of Chemical fertilizer and Pesticide Quality in Hunan,Changsha,Hunan 410014)

机构地区：[1]湖南化工研究院有限公司国家农药创制工程技术研究中心,湖南长沙410014 [2]湖南化肥农药质量监督检验授权站,湖南长沙410014

出　　处：《粮食科技与经济》2024年第1期85-88,共4页Food Science And Technology And Economy

基　　金：湖南省科技创新计划重点项目(2022NK2053)。

摘　　要：建立高效液相色谱法和气相色谱法,来测定肟菌酯原药有效成分的定量分析方法比较。用Agilent C_(18)柱,采用甲醇和水为流动相,通过二极管阵列检测器波长为250 nm;AE.SE-30毛细管柱,以邻苯二甲酸二环己酯为内标物,FID检测器分别对肟菌酯有效成分进行定量分析。高效液相色谱法和气相色谱法的线性相关系数分别为0.9999、0.9995,标准偏差分别为0.21、0.19,变异系数分别为0.21%、0.19%,平均回收率分别为99.74%和99.67%。通过比较,两种方法都能很好地用于肟菌酯原药的定量分析。Comparison of methods for quantitative analysis of the active ingredients of trifloxystrobin was determined by HPLC and gas chromatography.By a diode array detector wavelength at 250nm and FID detector,the active ingredient of trifloxystrobin was analyzed quantitatively With a Agilent C_(18) column and a AE.SE-30 Capillary column,using methanol and water as the mobile phase and dicyclohexyl phthalat as the internal standard.The results showed that the linear correlation coefficients of HPLC and gas chromatography were 0.9999 and 0.9995,the standard deviations were 0.21 and 0.19,the coefficient of variation was 0.21%and 0.19%,and the average recovery was 99.74%and 99.67%respectively.By comparison,the both methods are good for quantitative analysis of trifloxystrobin.

关 键 词：高效液相色谱 气相色谱 肟菌酯 

分 类 号：TQ450.7[化学工程—农药化工]