和枢消积方对人肝癌细胞系HepG2增殖凋亡的影响  

Effect of Heshu Xiaoji Prescription on the Proliferation and Apoptosis of HepG HepG2 Cell Line

作　　者：杨宗林 彭孟云 朱晓宁 汪静 YANG Zonglin;PENG Mengyun;ZHU Xiaoning;WANG Jing(College of Integrated Traditional Chinese and Western Medicine,Southwest Medical University,Sichuan Province,Luzhou 646000,China;Department of Hepatobiliary Diseases,Affiliated Hospital of Traditional Chinese Medicine of Southwest Medical University,Sichuan Province,Luzhou 646000,China)

机构地区：[1]西南医科大学中西医结合学院,四川泸州646000 [2]西南医科大学附属中医医院肝胆病科,四川泸州646000

出　　处：《中国中医药现代远程教育》2024年第12期145-148,共4页Chinese Medicine Modern Distance Education of China

基　　金：四川省科学技术厅重大科技专项【No.2019YFS0018】;四川省泸州市人民政府-西南医科大学科技战略合作项目【No.2019LZXNYDZ12】;西南医科大学附属中医医院国家中医临床研究基地建设单位科研项目【No.西南医大中医院〔2020〕33号】;四川省中医药管理局中医药科学技术研究专项【No.2020JC0145】;西南医科大学-西南医科大学附属中医医院联合项目【No.2018XYLH-030,2020XYLH-037】。

摘　　要：目的探讨和枢消积方对人肝癌细胞系HepG2增殖凋亡的影响,揭示可能的分子机制。方法使用和枢消积方作用于人肝癌细胞系HepG2,采用Cell Counting Kit-8(CCK-8)、平板克隆实验检测细胞增殖能力。采用蛋白质印迹法(Western blot)检测B淋巴细胞瘤-2基因(BCL-2)、BCL-2相关X蛋白(BAX)、丙型肝炎病毒(HCV)非结构5A蛋白(NS5A)反式激活基因13(NS5ATP13)及蛋白激酶B(AKT)/糖原合酶激酶(GSK)/雷帕霉素靶蛋白(MTOR)相关通路蛋白的表达水平。结果和对照组相比,和枢消积方各组可使HepG2细胞活性、增殖率显著降低(P<0.05);同时可上调BAX的蛋白表达,下调BCL-2及磷酸(p)-AKT、p-GSK、p-MTOR及NS5ATP13的表达(P<0.05),呈浓度依赖性。结论和枢消积方可以抑制HepG2细胞的增殖并诱导凋亡,这可能与和枢消积方抑制NS5ATP13的表达,继而抑制AKT/GSK/MTOR信号转导通路的活化有关。Objective To investigate the effect of Heshu Xiaoji prescription on the proliferation and apoptosis of human hepatoma cell line HepG2,and to explore the possible mechanism.Methods Heshu Xiaoji prescription was used to act on the human hepatoma cell line HepG2,CCK8,and plate clone assay were used to detect cell proliferation ability,respectively.Western blot was used to detect the expression changes of BCL-2,BAX,NS5ATP13 and AKT/GSK/MTOR-related pathway proteins.Results Compared with the control group,Heshu Xiaoji prescription can significantly reduce the activity,proliferation rate of HepG2 cells(P<0.05).Heshu Xiaoji prescription can up-regulate the protein expression of BAX,down-regulate the expression of BCL-2 and P-AKT,P-GSK,P-MTOR and NS5ATP13(P<0.05).Conclusion Heshu Xiaoji prescription can inhibit the proliferation of HepG2 cells and induce apoptosis,which may be related to the inhibition of the expression of NS5ATP13 and the activation of the AKT/GSK/MTOR signaling pathway.

关 键 词：和枢消积方 增殖凋亡 人肝癌细胞系HEPG2 实验研究 

分 类 号：R285[医药卫生—中药学]