枯草芽孢杆菌对湖羊瘤胃菌群和血清代谢物的影响  

Effect of Bacillus subtilis on rumen microbes and metabolites in Hu sheep

作　　者：宋淑珍[1] 刘俊斌 刘立山 宫旭胤[1] SONG Shuzhen;LIU Junbin;LIU Lishan;GONG Xuyin(Institute of Animal&Pasture Science and Green Agriculture,Gansu Academy of Agricultural Science,Lanzhou,Gansu 730070,China;College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所,甘肃兰州730070 [2]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《西北农林科技大学学报（自然科学版）》2024年第5期1-11,20,共12页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(32002203)。

摘　　要：【目的】利用16S rDNA高通量测序技术和LC-MS/MS非靶向代谢组学技术,研究短期和长期饲喂添加枯草芽孢杆菌日粮对育肥湖羊瘤胃内容物菌群和血清代谢物的影响。【方法】选择日龄((60±10)d)相近、体质量((19.51±2.07)kg/头)接近的湖羊断奶公羔36只,随机分为3组,分别为对照组(CON)、枯草芽孢杆菌Ⅰ组(BSN)和枯草芽孢杆菌Ⅱ组(BSH),每组12只羊。CON组饲喂基础饲粮,BSN组饲喂基础饲粮+100 mg/kg枯草芽孢杆菌,BSH组饲喂基础饲粮+200 mg/kg枯草芽孢杆菌。预试期10 d,正试期60 d,分别在正试期的第30天(短期)和第60天(长期)每组随机选择6只羊取瘤胃内容物和血清进行分析。【结果】①短期和长期饲喂添加枯草芽孢杆菌饲粮对湖羊瘤胃微生物丰富度指数Chao 1指数和多样性指数Shannon指数、Simpson指数均无显著影响(P>0.05)。湖羊瘤胃菌群在门水平上,短期饲喂BSN组的螺旋体菌门、迷踪菌门相对丰度较CON组显著降低(P<0.05),长期饲喂BSH组的变形菌门相对丰度显著升高(P<0.05);在属水平上,短期饲喂BSN组普雷沃氏菌属UCG-003相对丰度显著降低(P<0.05),而瘤胃球菌属1相对丰度显著升高(P<0.05)。长期饲喂BSN和BSH组湖羊普雷沃氏菌属UCG-003相对丰度显著降低(P<0.05)。②短期饲喂BSN和BSH组湖羊血清代谢物中L-苹果酸、D-2,3-二羟基丙酸、5-氨基水杨酸、2-氮杂环丁烷羧酸、棕榈酰溶血磷脂酸、1-磷酸果糖、核黄素均较CON组显著降低(P<0.05);BSN组5-磷酸脱氧核糖、丙烯酸、甘氨酰异亮氨酸、2-酮丁酸、1,2,4-偏苯三酚、β-D-半乳糖、丁酸乙酯均较CON组显著降低(P<0.05),而3′-磷酸腺苷较CON组显著升高(P<0.05),差异代谢物相关通路为辅因子的生物合成、核黄素代谢、磷酸转移酶系统等。长期饲喂BSH组10E,12Z-共轭亚油酸、N4-乙酰胞嘧啶、吡咯烷酮羧酸、9,10-环氧十八烷酸、肌酸酐均较CON和BSN组显著升高(P<0.05),差异代【Objective】This study assessed the short-term and long-term effects of Bacillus subtilis in diet on rumen microbes and serum metabolites in Hu sheep using 16S rDNA amplicon sequencing technology and LC-MS/MS non-targeted metabolomics technology.【Method】A total of 36 Hu lambs with similar age of(60±10)d and body weight of(19.51±2.07)kg were randomly divided into control group(CON),Bacillus subtilis groupⅠ(BSN)and Bacillus subtilis groupⅡ(BSH),with 12 lambs in each group.CON was fed the basal diet,BSN was fed the basal diet with 100 mg/kg Bacillus subtilis,and BSH was fed the basal diet with 200 mg/kg Bacillus subtilis.The pre-test period was 10 days and the test period was 60 days.Six sheep in each group were randomly selected to collect rumen contents and serum for analysis at days 30 and 60.【Result】①Feeding Bacillus subtilis for short-term(30 days)and long-term(60 days)had no significant effect on microbial richness index of Chao 1 and Shannon and Simpson diversity indexes of rumen contents of Hu sheep(P>0.05).At the level of phylum,relative abundances of Spirochetes and Elusimicrobia were significantly lower in group BSN for short-term feeding(P<0.05),and that of Proteobacteria was significantly higher in group BSH for long-term feeding(P<0.05).At the level of genus,relative abundance of Prevotellaceae UCG-003 decreased significantly(P<0.05),while that of Ruminococcus 1 increased significantly in group BSN for short-term feeding(P<0.05).The relative abundances of Prevotellaceae UCG-003 decreased significantly in group BSN and BSH for long-term feeding(P<0.05).②Among serum metabolites,L-malic acid,D-2,3-dihydroxypropanoic acid,mesalazine,2-azetidinecarboxylic acid,lyso PA(16:0/0:0),fructose-1P and riboflavin decreased significantly in group BSN and group BSH for short-term feeding(P<0.05).The deoxyribose 5-phosphate,caprylic acid,glycyl-Isoleucine,2-ketobutyric acid,benzene-1,2,4-triol,beta-D-galactose and ethyl butyrate decreased significantly(P<0.05),while 3′-AMP increased significantly

关 键 词：枯草芽孢杆菌 瘤胃菌群 血清代谢物 湖羊 

分 类 号：S816.73[农业科学—饲料科学]