蛇伤胶囊对竹叶青蛇伤兔血小板活化功能影响的研究  

Study on the effect of Sheshang Capsule on platelet activation function in rabbits injured by Trimeresurus stejnegeri

作　　者：邵丹[1] 吴晖[1] 梁志奇[1] 吴丹[1] 陈宏杰[1] 王榕[1] SHAO Dan;WU Hui;LIANG Zhiqi;WU Dan;CHEN Hongjie;WANG Rong(Emergency Department,Fujian People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou 350004,China)

机构地区：[1]福建中医药大学附属人民医院急诊科,福建福州350004

出　　处：《中国医药科学》2024年第7期25-29,共5页China Medicine And Pharmacy

基　　金：福建省自然科学基金(2020J011047)。

摘　　要：目的研究蛇伤胶囊对竹叶青蛇伤兔血小板活化功能的影响。方法选取24只新西兰大白兔随机分为正常对照组、模型组、蛇伤胶囊组,每组各8只,雌雄各半,根据前期研究方法模型组和蛇伤胶囊组以兔耳缘静脉注射0.75 mg/kg竹叶青蛇毒液建立竹叶青蛇伤兔模型,正常对照组注射等量生理盐水。注射6 h后予正常对照组和模型组10 ml/(kg·d)生理盐水灌胃,蛇伤胶囊组予10 ml/(kg·d)蛇伤胶囊所配药液灌胃。以上3组连续灌胃1周后兔耳缘静脉采血5 ml,血小板分离提取后应用实时荧光定量聚合酶链式反应技术(qPCR)检测各组血小板活化指标αⅡb整合素(CD41)、整合素β链Ⅲ(CD61)、血小板P选择素(CD62P)、溶酶体颗粒膜糖蛋白(CD63)信使核糖核酸(mRNA)表达,酶联免疫吸附试验(ELISA)检测膜糖蛋白Ⅱb/Ⅲa(GPⅡb/Ⅲa)水平。分析比较各组血小板活化指标CD41、CD61、CD62P、CD63 mRNA表达及GPⅡb/Ⅲa水平差异。结果各组血小板细胞CD41、CD61、CD62P、CD63 mRNA表达及GPⅡb/Ⅲa水平组间比较,差异均有显著统计学意义(P<0.01)。两两组间比较,与正常对照组比较,模型组CD41、CD61、CD62P、CD63 mRNA表达及GPⅡb/Ⅲa水平显著降低,差异有显著统计学意义(P<0.01),蛇伤胶囊组CD41、CD61、CD62P、CD63 mRNA表达显著降低,差异有显著统计学意义(P<0.01),GPⅡb/Ⅲa水平显著降低,差异有统计学意义(P<0.05);与模型组比较,蛇伤胶囊组CD41、CD61、CD62P、CD63 mRNA表达显著增加,差异有显著统计学意义(P<0.01),GPⅡb/Ⅲa水平显著增加,差异有统计学意义(P<0.05)。结论竹叶青蛇伤时血小板活化功能受抑制,蛇伤胶囊可通过提高CD41、CD61、CD62P、CD63、GPⅡb/Ⅲa水平,减轻血小板活化功能抑制,改善血小板活化功能。Objective To study the effect of Sheshang Capsule on platelet activation function in rabbits injured by Trimeresurus stejnegeri.Methods A total of 24 New Zealand white rabbits were randomly divided into a normal control group,a model group,and a Sheshang Capsule group,with 8 rabbits in each group,half male and half female.According to the previous research method,the model group and the Sheshang Capsule group were injected with 0.75 mg/kg Trimeresurus stejnegeri’s venom into the rabbit ear vein to establish a rabbit injured by Trimeresurus stejnegeri model,while the normal control group was injected with an equal amount of physiological saline.After 6 hours of injection,the normal control group and the model group were gavaged with 10 ml/(kg·d)physiological saline,while the Sheshang Capsule group was gavaged with 10 ml/(kg·d)solution with Sheshang Capsule content.After continuous gavage of the above three groups for one week,5 ml of blood was collected from the rabbit ear vein respectively.After platelet separation and extraction,real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect platelet activation indicators,i.e.,integrin α Ⅱb(CD41),integrin β Ⅲ chain(CD61),platelet P-selectin(CD62P),and lysosomal membrane glycoprotein(CD63)messenger RNA(mRNA)expression,and enzyme-linked immunosorbent assay(ELISA)to detect membrane glycoprotein Ⅱb/Ⅲa(GPⅡb/Ⅲa)levels in each group.The differences in platelet activation indicators CD41,CD61,CD62P,CD63mRNA expression,and GPⅡb/Ⅲa levels was analyzed and compared among different groups.Results There were significant differences in CD41,CD61,CD62P,CD63 mRNA expression and GPⅡb/Ⅲa levels between groups(P<0.01).Compared with the normal control group,the expression of CD41,CD61,CD62P,CD63 mRNA,and GPⅡb/Ⅲa levels in the model group were significantly reduced,with the differences were statistically significant(P<0.01),while the expression of CD41,CD61,CD62P and CD63 mRNA in the Sheshang Capsule group was significantly reduced

关 键 词：竹叶青蛇 蛇伤胶囊 模型建立 血小板活化 凝血障碍 

分 类 号：R269[医药卫生—中西医结合]