基于网络药理学探讨郁火咳嗽方治疗咳嗽的作用机制  

Exploring the mechanism of Yuhuo cough formula in treating cough based on network pharmacology

作　　者：余娇 黄学宽[1] 王辉[2] YU Jiao;HUANG Xuekuan;WANG Hui(College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400041,China;Department of Pulmonary Disease,Beibei Traditional Chinese Medical Hospital,Chongqing 400700,China)

机构地区：[1]重庆医科大学中医药学院,重庆400041 [2]重庆市北碚区中医院肺病科,重庆400700

出　　处：《中国医药科学》2024年第7期90-95,166,共7页China Medicine And Pharmacy

基　　金：重庆市市级中医重点专科建设项目(渝中医[2023]17号)。

摘　　要：目的利用网络药理学构建郁火咳嗽方药效成分、咳嗽、靶点及通路间的网络关系,研究郁火咳嗽方治疗咳嗽的作用机制。方法利用中药系统药理学数据库分析平台(TCMSP)查询郁火咳嗽方的药效成分及对应靶点,从Drugbank、GeneCards、PharmGkb、Therapeutic Target Database(TTD)数据库获取咳嗽相关靶点,使用在线软件基迪奥生(genedenovo)筛选药物与疾病作用的交集靶点;基于DAVID数据库对相关基因靶点进行基因本体(GO)功能及京都基因和基因组百科全书(KEGG)通路富集分析。利用STRING 11.0在线数据库构建蛋白质-蛋白质相互作用(PPI)网络;用Cytoscape软件绘制活性成分-疾病靶点关系网络。结果郁火咳嗽方的药效成分有205个,以黄酮类成分为主;其治疗咳嗽的关键靶点有细胞色素P4503A4酶(CYP3A4)、组蛋白去乙酰化酶(HDAC)、雌激素受体1(ESR1)等。GO富集分析后得到与生物过程(BP)相关的有有机物质代谢过程、对化学物质反应等,与细胞组分(CC)相关的有细胞器、细胞质、膜界细胞器等,与分子功能(MF)相关的有离子结合、有机环状化合物、杂环化合物结合等;KEGG通路主要涉及代谢通路、癌症通路、类固醇激素生物合成、5-羟色胺突触等。结论郁火咳嗽方的主要成分以黄酮类成分为主,主要通过抑制炎症因子的表达从而达到抗炎止咳的作用。Objective To construct a network relationship between the pharmacological components,cough,targets,and pathways of Yuhuo cough formula using network pharmacology,and to study the mechanism of action of Yuhuo cough formula in relieving cough.Methods The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)was used to search for the pharmacological components and corresponding targets of the Yuhuo cough formula.Cough-related targets were obtained from Drugbank,GeneCards,PharmaGkb,and Therapeutic Target Database(TTD),and online software genedenovo was used to screen the intersection targets of the drug and disease effects.Based on the DAVID database,gene ontology(GO)function and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were conducted on relevant gene targets.Protein-protein interaction networks were established using the STRING 11.0 online database.The active ingredient-disease target relationship networks were plotted using Cytoscape.Results There are 205 active ingredients in the Yuhuo cough formula,mainly flavonoids.The key targets for treating cough include cytochrome P4503A4(CYP3A4),histone deacetylase(HDAC),estrogen receptor 1(ESR1),etc.After GO enrichment analysis,it is found that there are organic matter metabolism processes and chemical reactions related to biological processes(BP),organelles,cytoplasm and membrane boundary organelles related to cellular components(CC),and ion binding,organic cyclic compounds and heterocyclic compounds binding related to molecular functions(MF).The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway mainly involves metabolic pathways,pathways in cancer,steroid hormone biosynthesis,and serotonin synapse.Conclusion The main components of the Yuhuo cough formula are flavonoids,achieving anti-inflammatory and cough-relieving effects mainly by inhibiting the expression of inflammatory factors.

关 键 词：郁火咳嗽方 咳嗽 作用机制 网络药理学 

分 类 号：R256.11[医药卫生—中医内科学]