抗癌药物喜树碱的超声辅助PEG400绿色提取工艺研究  

Study on Ultrasonic-Assisted PEG400 Extraction of Camptothecin from Camptotheca Acuminata Seed

作　　者：徐铖 魏巍[1] 屈保玉 顾成波[1] 袁肖寒[2] Xu Cheng(Key Laboratory of Forest Plant Ecology of Ministry of Education,Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-Based Active Substances,Engineering Research Center of Forest Bio-Preparation of Ministry of Education,College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin Heilongjiang 150040)

机构地区：[1]东北林业大学化学化工与资源利用学院,森林植物生态学教育部重点实验室,林业生物制剂教育部工程研究中心,黑龙江省林源活性物质生态利用重点实验室,哈尔滨150040 [2]东北农业大学生命科学与生物技术研究中心,哈尔滨150030

出　　处：《黑龙江医药》2024年第2期249-253,共5页Heilongjiang Medicine journal

基　　金：“十四五”国家重点研发计划项目子课题“喜树碱类新型医药中间体及其绿色制备技术”(编号:2022YFD2200805-03)。

摘　　要：目的:建立使用表面活性剂PEG400结合超声辅助从喜树种子中提取抗癌药物喜树碱的绿色提取工艺。方法:通过对不同表面活性剂对喜树碱的溶解度的考察,确定适合喜树碱提取的表面活性剂及其最佳浓度,同时对提取过程中的液料比、超声功率、超声时间、温度进行单因素实验和正交试验。结果:采用13%的PEG400水溶液结合超声提取喜树碱的最佳工艺条件为液料比90∶1、超声功率540W、超声时间90min、温度25℃,在此条件下的喜树碱提取率为1174.59μg/g。结论:使用PEG400水溶液成功提取了喜树种子中的喜树碱,喜树碱的提取率为1174.59μg/g。Objective:To establish an organic-free extraction method by using surfactants combined with ultrasonic-assisted extraction of camptothecin from Camptotheca acuminata seeds.Methods:The surfactant was screened and its optimal concentration was determined by the solubility of surfactant in camptothecin.The single factor experiment and orthogonal experiment were conducted on the liquid-solid ratio,ultrasonic power,ultrasonic time and temperature in the extraction process.Results:The optimal extraction conditions for camptothecin with 13%PEG400 aqueous solution were liquid-solid ratio of 90∶1,ultrasonic power of 540W,ultrasonic time of 90min and temperature of 25°C.Under these conditions,the extraction rate of camptothecin was 1174.59μg/g.Conclusion:Camptothecin was successfully extracted from Camptotheca acuminata seeds with PEG400 aqueous solution,and the extraction rate was 1174.59μg/g.

关 键 词：表面活性剂 超声波 提取 喜树碱 

分 类 号：TQ461[化学工程—制药化工] R284.2[医药卫生—中药学]