淹水胁迫对西蒙得木幼苗根际土壤真菌多样性、群落结构和功能组成的影响  

Effects of Water Logging Stress on Fungal Diversity,Community Structure and Functional Composition in Rhizosphere Soils of Simmondsia chinensis Seedlings

作　　者：梁向 李明蔓 何鹏 覃纤纤 贝恩光 李继超 梁猛强 曾敏 程飞[1] Liang Xiang;Li Mingman;He Peng;Qin Xianxian;Bei Enguang;Li Jichao;Liang Mengqiang;Zeng Min;Cheng Fei(College of Forestry,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Vocational&Technical College,Nanning,Guangxi 530226,China;Experimental Center of Tropical Forestry,Chinese Academy of Forestry,Chongzuo,Guangxi 532600,China;State-owned Guangmingshan Forest Farm of Mashan County,Mashan,Guangxi 530601,China)

机构地区：[1]广西大学林学院,广西南宁530004 [2]广西职业技术学院,广西南宁530226 [3]中国林业科学研究院热带林业实验中心,广西崇左532600 [4]国营马山县光明山林场,广西马山530601

出　　处：《广西林业科学》2024年第2期170-177,共8页Guangxi Forestry Science

基　　金：国家自然科学基金项目(31600493);广西自然科学基金面上项目(2017GXNSFAA198007)。

摘　　要：为了解西蒙得木(Simmondsia chinensis)在淹水胁迫下根际土壤真菌群落的变化规律,以田间持水量的70%为对照(CK),设置轻度、中度和重度淹水胁迫处理,对2月龄西蒙得木幼苗进行7天淹水胁迫,采用高通量测序技术对不同淹水胁迫处理的西蒙得木幼苗根际土壤真菌多样性、群落结构和功能组成进行分析。结果表明,西蒙得木幼苗根际土壤真菌以子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)和壶菌门(Chytridiomycota)为主。轻度、中度淹水胁迫处理的根际土壤真菌多样性和丰富度与CK差异不显著,重度淹水胁迫处理的根际土壤真菌多样性和丰富度高于其他处理。NMDS和PCA分析结果表明,轻度和中度淹水胁迫处理的根际土壤真菌群落结构和功能组成较相似,重度淹水胁迫处理的根际土壤真菌群落结构和功能组成与其他处理差异明显。腐生营养型真菌在不同淹水胁迫处理的根际土壤真菌群落中占主导。在我国南方种植西蒙得木时,在苗期要避免积水,并结合其他栽培和防治措施进行综合治理,防止病害发生和蔓延。In order to understand changing laws of fungal communities in rhizosphere soils of Simmondsia chinensis under water logging stress,70%of field capacity was used as control(CK),and mild,moderate and severe water logging stress treatments were set up.Two-month-old S.chinensis seedlings were subjected to 7 days of water logging stress.Diversities,community structures and functional compositions of fungi in rhizosphere soils of S.chinensis seedlings in different water logging stress treatments were analyzed using highthroughput sequencing technology.Results showed that dominant fungi in rhizosphere soils of S.chinensis seedlings were Ascomycota,Basidiomycota,Mortierellomycota,and Chytridiomycota.Diversities and richness of fungi in rhizosphere soils in mild and moderate water logging stress treatments did not significantly differ from CK,while diversities and richness of fungi in rhizosphere soils in severe water logging stress treatment were significantly higher than those of other treatments.Results of NMDS and PCA analysis indicated that community structures and functional compositions of fungi in rhizosphere soils in mild and moderate water logging stress treatments were similar,which in severe water logging stress treatment showed significant differences from other treatments.Saprotroph fungi dominated in fungal communities in rhizosphere soils in different water logging stress treatments.When S.chinensis was cultivated in southern China,water logging should be avoided during seedling stage.Comprehensive management should be implemented combining other cultivation and control measures to prevent occurrence and spread of diseases.

关 键 词：淹水胁迫 根际土壤 真菌群落结构 功能组成 西蒙得木 

分 类 号：S723.6[农业科学—林木遗传育种]