创伤性膝骨关节炎患者血清CX3CL1、Apelin-13与炎症指标的相关性及预后价值  

Correlation and prognostic value of serum CX3CL1,Apelin-13 and inflammatory markers in patients with traumatic knee osteoarthritis

作　　者：吕新凯 李光磊[1] 段强民[1] 王宝鹏[1] 张平[1] 孙全省 张冰[1] LYU Xinkai;LI Guanglei;DUAN Qiangmin;WANG Baopeng;ZHANG Ping;SUN Quansheng;ZHANG Bing(First Department of Orthopedics,People′s Hospital of Linzi District of Zibo,Zibo,Shandong 255400,China)

机构地区：[1]山东省淄博市临淄区人民医院骨科一病区,山东淄博255400

出　　处：《国际检验医学杂志》2024年第9期1126-1130,共5页International Journal of Laboratory Medicine

摘　　要：目的 探索创伤性膝骨关节炎(PTOA)患者血清趋化因子CX3CL1及脂肪细胞因子-13(Apelin-13)的表达水平,并分析二者与炎症指标的相关性及预后价值。方法 选取2020年1月至2022年2月在该院确诊的65例PTOA患者作为试验组,选取同期来该院体检的55例体检健康者作为对照组。通过酶联免疫吸附试验(ELISA)检测血清CX3CL1与Apelin-13的表达水平。Pearson法相关性分析血清CX3CL1与Apelin-13与炎症指标的相关性。绘制受试者工作特征(ROC)曲线分析CX3CL1与Apelin-13对PTOA患者预后的诊断价值。多因素Logistic回归分析PTOA患者预后不良的影响因素。结果 与对照组相比,试验组中白细胞介素-6(IL-6)、瘦素、降钙素原(PCT)、超敏C反应蛋白(hsCRP)、肿瘤坏死因子-α(TNF-α)、CX3CL1的表达水平显著升高,差异有统计学意义(P<0.05),Apelin-13的表达水平显著下降,差异有统计学意义(P<0.05);Pearson法相关性分析显示,CX3CL1与IL-6、瘦素、TNF-α呈正相关(r=0.528、0.602、0.511,P<0.001)。Apelin-13与IL-6呈负相关(r=-0.541,P<0.001);多因素Logistic回归分析显示IL-6、瘦素、TNF-α、CX3CL1与Apelin-13是PTOA患者预后不良的影响因素(P<0.05);ROC曲线下面积(AUC)分析显示,与血清CX3CL1和Apelin-13单独指标相比,CX3CL1联合Apelin-13预测预后不良的AUC更大(Z_(联合)-CX3CL1=2.010,P=0.044;Z_(联合)-Apelin-13=2.091,P=0.036)。结论 PTOA患者血清CX3CL1表达水平显著升高,Apelin-13表达水平显著降低,CX3CL1与Apelin-13是PTOA预后不良的影响因素,二者联合检测对于PTOA的预后提供帮助。Objective To explore the expression levels of serum chemokine CX3CL1 and adipocytokine-13(Apelin-13)in patients with post-traumatic knee osteoarthritis(PTOA),and analyze their correlation with inflammatory indicators and prognostic value.Methods A total of 65 patients with PTO diagnosed in the hospital from January 2020 to February 2022 were selected as the experimental group,while 55 healthy volunteers who came to the hospital for physical examination were collected as the control group.Enzyme linked immunosorbent assay(ELISA)was drawn to detect the expression levels of serum CX3CL1 and Apelin-13.Pearson correlation method was applied to analyze the correlation between serum CX3CL1 and Apelin-13 with inflammatory markers.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of CX3CL1 and Apelin-13 for the prognosis of patients with PTOA.Multivariate Logistic regression was applied to analyze the influencing factors of poor prognosis in patients with PTOA.Results Compared with the control group,the expression levels of interleukin-6(IL-6),leptin,procalcitonin(PCT),hypersensitive C-reactive protein(hsCRP),tumor necrosis factor-α(TNF-α)and CX3CL1 in the experimental group were obviously increased,and the differences were statistically significant(P<0.05),while the expression level of Apelin-13 was obviously decreased,and the difference was statistically significant(P<0.05).Pearson correlation analysis showed that CX3CL1 was obviously positively correlated with IL-6,leptin,and TNF-α(r=0.528,0.602,0.511,P<0.001).There was an obvious negative correlation between Apelin-13 and IL-6(r=-0.541,P<0.001).Multivariate Logistic regression analysis showed that IL-6,leptin,TNF-α,CX3CL1,and Apelin-13 were the influencing factors for poor prognosis in patients with PTOA(P<0.05).The area under the curve(AUC)showed that,compared to the serum CX3CL1 and Apelin-13 individual indicators,the combination of CX3CL1 and Apelin-13 had a larger AUC in predicting poor prognosis(Z_(combination-Apelin-13)=

关 键 词：创伤性膝骨关节炎 CX3CL1 脂肪细胞因子-13 预后 

分 类 号：R684.3[医药卫生—骨科学]