盐碱胁迫下湖南稷子苗期根系分泌物代谢组学  

Metabolomics analysis of root exudates in Echinochloa frumentacea seedling stage under saline⁃alkali stress

作　　者：张杨 曹靖[3] 李广[2] 姜世腾[2] 于倩 聂豪杰[1] 李林傲 朱林 ZHANG Yang;CAO Jing;LI Guang;JIANG Shiteng;YU Qian;NIE Haojie;LI Lin′ao;ZHU Lin(School of Ecology and Environmental Sciences,Ningxia University/Breeding Base for State Key Lab.of Land Degradation and Ecological Restoration in Northwestern China/Key Lab.of Restoration and Reconstruction of Degraded Ecosystems in northwestern China of Ministry of Education,Yinchuan 750021,China;Gansu Analysis and Research Center,Lanzhou 730030,China;School of Ecology Lanzhou University,Lanzhou 730030,China;School of Agriculture Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学生态环境学院/西北土地退化与生态恢复国家重点实验室培育基地/西北退化生态系统恢复与重建教育部重点实验室,银川750021 [2]甘肃省分析测试中心,兰州730030 [3]兰州大学生态学院,兰州730030 [4]宁夏大学农学院,银川750021

出　　处：《生态学报》2024年第8期3540-3549,共10页Acta Ecologica Sinica

基　　金：宁夏自然科学基金项目(2022AAC03086);甘肃省自然科学基金项目(21JR7RA760);宁夏回族自治区农业育种专项(2019NYYZ0401)。

摘　　要：盐碱胁迫下植物根系分泌物包含丰富生化信息并具有重要生态作用。为了探讨耐盐碱牧草湖南稷子(Echinochloa frumentacea)在盐碱胁迫下根系分泌物组成,揭示其在盐碱胁迫下的生理及生态作用,以湖南稷子为试验对象,在人工气候室开展水培试验,并在苗期分别进行中性盐(NaCl+Na_(2)SO_(4)100 mmol/L)、碱性盐(NaCl+NaHCO_(3)100 mmol/L)和碱(Na_(2)CO_(3)+NaHCO_(3)50 mmol/L)处理。在处理3 d后,利用液质联用仪(LC⁃MS/MS)检测对照组和处理组根系分泌物的化合物成分。结果表明,盐碱胁迫下湖南稷子根系分泌物共有334种化合物。依据正交偏最小二乘法判别分析(OPLS、|DA),重要值(VIP)得分及t检验的P值,发现对照比SaSo100(碱性盐处理100 mmol/L),对照比Soda50(碱处理50 mmol/L)和对照比Salt100(中性盐处理100 mmol/L)分别有22、15和21个差异根系分泌物。其中碱性盐和碱处理下根系分泌物组成相近,包括脂质、酚酸,生物碱,苯酞类,氨基糖,萜类,醌类,氨基酸及其衍生物;中性盐处理下有脂质、酚酸,生物碱,苯酞类,萜类。京都基因与基因组百科全书注释及富集发现,盐碱胁迫下根系分泌物不仅含有三羧酸循环代谢产生的碳水化合物、核苷酸,氨基酸,脂肪酸,类脂和维生素等物质,而且与瓦博格效应、膜运输,信号传导以及遗传信息处理等途径有关。研究表明,湖南稷子通过根系分泌物渗出,调节自身代谢物浓度,加强或改变碳同化、呼吸作用、信号传导等提高对盐碱胁迫的适应性。Root exudates of plants under saline⁃alkali stress contain abundant biochemical information and play an important ecological role.In order to explore the composition of root secretions of salt⁃tolerant pasture grass Echinochloa frumentacea under salt⁃alkali stress,and to reveal its physiological and ecological functions under salt⁃alkali stress.A hydroponic experiment was carried out on Echinochloa frumentacea in an artificial climate chamber,with neutral salt(NaCl+Na_(2)SO_(4)100 mmol/L),alkaline salt(NaCl+NaHCO_(3)100 mmol/L)and alkali(Na2 CO_(3)+NaHCO_(3)50 mmol/L)treatments at the seedling stage.After 3 days of treatment,the composition of compound of root exudates in the control and treatment groups was detected by LC⁃MS/MS.The results showed that there were 334 compounds in root exudates of Echinochloa frumentacea under salt⁃alkali stress.According to the VIP score of OPLS⁃DA and the P value of ttest,22,15 and 21 differential root exudates were found for control⁃vs⁃saso100,control⁃vs⁃soda50 and control⁃vs⁃salt100,respectively.The compositions of root exudates under alkaline salt and alkali treatments were similar,including lipids,phenolic acids,alkaloids,phthalides,amino sugars,terpenoids,quinones,amino acids and their derivatives;There are lipids,phenolic acids,alkaloids,phthalides,terpenoids under neutral salt treatment.KEGG annotation and enrichment showed that root exudates under salinealkali stress not only contained carbohydrates,nucleotides,amino acids,fatty acids,lipids and vitamins produced by tricarboxylic acid cycle metabolism,but also were related to Warburg effect,membrane transport,signal transduction and genetic information processing.Studies have shown that Echinochloa frumentacea improved its adaptability to saline⁃alkali stress through exudation of root exudates,regulating its metabolite concentration,enhancing or changing carbon assimilation,respiration,and signal transduction.

关 键 词：盐碱胁迫 湖南稷子(Echinochloa frumentacea) 根系分泌物 代谢组学 

分 类 号：S544.9[农业科学—作物学]