环境温度和饲粮金荞麦提取物对蛋鸡肝脏超微结构及线粒体抗氧化功能的影响  

Effects of ambient temperature and dietary FagoPyrum dibotrys Hara extract on liver ultrastructure and mitochondrial antioxidant function of laying hens

作　　者：刘凤丹 张蓉 陈光吉 聂朝松 吴建高 潘凤清 尚以顺 李世歌[1] 陈彦伶 龙忠富 班宋智 LIU Fengdan;ZHANG Rong;CHEN Guangji;NIE Chaosong;WU Jiangao;PAN Fengqing;SHANG Yishun;LI Shige;CHEN Yanling;LONG Zhongfu;BAN Songzhi(Guizhou Institute of prataculture,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou Province 550006,China;Dafang County Agriculture and Rural Bureau,Dafang,Guizhou Province 550707,China;Agricultural and Rural Bureau of Libo County,Libo,Guizhou Province 558400,China;Changshun County Songzhi farm,Changshun,Guizhou Province 550707,China)

机构地区：[1]贵州省农业科学院草业研究所,贵州贵阳550006 [2]大方县农业农村局,贵州大方550707 [3]荔波县农业农村局,贵州荔波558400 [4]长顺县送智养殖场,贵州长顺550707

出　　处：《中国饲料》2024年第9期26-33,共8页China Feed

基　　金：黔科合支撑[2021]一般157号;黔科合支撑[2021]一般152;黔科合服企[2022]004,国家牧草产业技术体系黔南综合试验站。

摘　　要：试验旨在研究饲粮金荞麦提取物(FDR)对不同温度条件下的长顺绿壳蛋鸡肝脏组织超微结构和线粒体抗氧化功能的影响。采用3×4的双因子完全随机试验设计,将192只鸡分为22℃常温、28℃中温和33℃高温以及4个FDR添加水平:0、200、400、800 mg/kg,共12个处理组。每个处理4个重复,每个重复4只鸡。将试验鸡只按照温度梯度分别安排到3个独立的温控鸡舍,于试验结束采集肝脏样品用于超微结构及线粒体抗氧化指标检测。结果显示:(1)33℃高温下蛋鸡肝脏组织超微结构损伤最严重,高温处理下不同浓度FDR添加肝脏组织超微结构损伤有所减轻,添加400 mg/kg组损伤程度最轻;(2)33℃环境条件下,谷胱甘肽过氧化物酶(GSH-Px)活性和超氧化物歧化酶(SOD)活性分别下降了13%和6.1%,丙二醛(MDA)含量和蛋白质羰基(PC)含量分别上升了17.3%和13.9%,FDR添加与温度的影响呈相反趋势,环境温度和FDR两因子添加对蛋鸡GSH-Px活性、SOD活性、MDA含量和PC含量均有显著的互作影响(P <0.05);(3)饲粮中添加FDR可上调蛋鸡肝脏中Nrf2信号通路与Trx2系统相关基因m RNA表达,但差异不显著(P> 0.05),环境温度与FDR两因子的添加对蛋鸡肝脏各个基因mRNA相对表达量有显著的互作影响(P <0.05)。综上所述,蛋鸡长时间暴露在高温环境下肝脏抗氧化能力降低,饲粮中添加400 mg/kg或更高含量的金荞麦提取物可缓解高温环境下蛋鸡的肝脏组织超微结构损伤,以及提高蛋鸡肝脏线粒体抗氧化能力。The experiment aimed to investigate the effects of FagoPyrum dibotrys Hara extract(FDR)on the liver tissue ultrastructure and mitochondrial antioxidant function of Changshun green shell laying hens under different temperature conditions.192 laying hens were randomly into 12 groups with 4 replicates,each replicate 4 chickens.A 3×4 factorial design was adopted.Each process had 4 replicates,with 4 chickens per replicate.Arrange the experimental chickens into three independent temperature controlled chicken coops according to temperature gradients,and collect liver samples at the end of the experiment for ultrastructural and mitochondrial antioxidant index detection.The results showed that:(1)Under high temperature of 33℃,the liver tissue ultrastructure damage of laying hens was the most severe.Under high temperature treatment,the liver tissue ultrastructure damage was alleviated by adding different concentrations of FDR,while adding 400 mg/kg had the least damage.(2)Under 33℃environmental conditions,glutathione peroxidase(GSH-Px)activity and superoxide dismutase(SOD)activity decreased by 13%and 6.1%,respectively,while malondialdehyde(MDA)content and protein carbonyl(PC)content increased by 17.3%and 13.9%,respectively.The effect of FDR addition on temperature showed an opposite trend.The addition of environmental temperature and FDR had a significant interaction effect on GSH-Px activity,SOD activity,MDA content and PC content in laying hens(P<0.05);(3)Adding FDR to the diet upregulated the mRNA expression of Nrf2 signaling pathway and Trx2 system related genes in the liver of laying hens,but not significantly(P>0.05).The addition of environmental temperature and FDR had a significant interaction effect on the relative mRNA expression of various genes in the liver of laying hens(P<0.05).In summary,long-term exposure to high temperature reduces the antioxidant capacity of the liver of laying hens.Adding 400 mg/kg or higher content of buckwheat extract to the diet could alleviate the ultrastructural damage of the

关 键 词：金荞麦提取物 环境高温 长顺绿壳蛋鸡 抗氧化 免疫 

分 类 号：S815[农业科学—畜牧学]