小站稻秸秆降解复合菌系的筛选与产酶条件优化  

Screening and optimization of enzyme-producing conditions of Xiaozhan rice straw degradation composite microbial system

作　　者：白狄纯 洪梓萌 郭娟娟 李佳怡 王金龙 BAI Dichun;HONG Zimeng;GUO Juanjuan;LI Jiayi;WANG Jinlong(Tianjin Agricultural University,College of Agriculture,Resources and Environment,Xiqing,Tianjin 300392,China)

机构地区：[1]天津农学院农学与资源环境学院,天津西青300392

出　　处：《中国饲料》2024年第9期65-71,共7页China Feed

基　　金：国家自然科学基金(31770505);天津农学院大学生创新创业训练项目(202210061125)。

摘　　要：为寻找一种能够科学高效地将小站稻秸秆用于秸秆还田的方法,并将其作为一种储备技术,本研究通过测定已有6组秸秆降解复合菌系的羧甲基纤维素酶活力和滤纸酶活力,筛选出2组复合菌系,并通过正交试验进行产酶条件的优化,以探索解决小站稻秸秆清洁生产技术的应用问题,保障小站稻的未来绿色可持续发展,为小站稻秸秆高效处理的研究提供理论基础和技术支持。本研究结果表明:复合菌系B和复合菌系F的综合酶活力均高于其他复合菌系;复合菌系B羧甲基纤维素酶最佳发酵条件为25℃,pH 8.0,接种量2%,温度为酶活力主要影响因素;复合菌系B滤纸酶最佳发酵条件为20℃,pH 9.0,接种量2%,pH为酶活力主要影响因素;复合菌系F羧甲基纤维素酶最佳发酵条件为25℃,pH 8.0,接种量2%,温度为酶活力主要影响因素;复合菌系F滤纸酶最佳发酵条件为20℃,pH9.0,接种量2%,接种量为酶活力主要影响因素。In order to find a scientific and efficient method for using Xiaozhan rice straw for straw returning to the field,and to use it as a reserve technology,this study determined the carboxymethyl cellulase activity and filter paper enzyme activity of six existing straw degrading composite bacterial strains,screened two composite bacterial strains,and optimized the enzyme production conditions through orthogonal experiments to explore and solve the application problems of Xiaozhan rice straw clean production technology,ensure the future green and sustainable development of Xiaozhan rice,and provide theoretical basis and technical support for the research on efficient treatment of Xiaozhan rice straw.The results of this study indicated that the comprehensive enzyme activity of composite strain B and composite strain F was higher than that of other composite strains;The optimal fermentation conditions for compound strain B carboxymethyl cellulase were 25℃,pH 8.0,inoculation amount of 2%,and temperature was the main influencing factor on enzyme activity;The optimal fermentation conditions for composite strain B filter paper enzyme were 20℃,pH 9.0,inoculation amount of 2%,and pH was the main influencing factor on enzyme activity;The optimal fermentation conditions for compound strain F carboxymethyl cellulase were 25℃,pH 8.0,inoculation amount of 2%,and temperature was the main influencing factor on enzyme activity;The optimal fermentation conditions for composite strain F filter paper enzyme were 20℃,pH 9.0,and 2%inoculation,which was the main influencing factor on enzyme activity.

关 键 词：小站稻 秸秆降解 复合菌系 筛选 产酶条件优化 

分 类 号：S816.3[农业科学—饲料科学]