单细胞蛋白质成像方法进展  

Progress of single-cell protein imaging methods

作　　者：姚春露 张惟杰 张云龙 邓朝霞 王梦灵 张左玲 王琛 宋沁馨[1] 邹秉杰 YAO Chunlu;ZHANG Weijie;ZHANG Yunlong;DENG Zhaoxia;WANG Mengling;ZHANG Zuoling;WANG Chen;SONG Qinxin;ZOU Bingjie(Key Laboratory of Drug Quality Control and Pharmacovigilance of Ministry of Education,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室,南京210009

出　　处：《中国药科大学学报》2024年第2期147-157,共11页Journal of China Pharmaceutical University

基　　金：国家自然科学基金项目(No.82173780);江苏省重点研发计划社会发展面上项目(No.BE2023843);药物质量与安全预警教育部重点实验室资助项目(No.DQCP20/21MS04);江苏省研究生科研与实践创新计划项目(No.KYCX23-0854);大学生创新创业训练计划项目(No.2023103161187)。

摘　　要：单细胞蛋白质的差异化表达及其亚细胞定位同机体的生理状态和病理机制密切相关。单细胞蛋白质原位成像方法的发展为空间单细胞蛋白质组学研究以及单细胞蛋白图谱绘制提供了强有力的工具。本文对近几年开发的基于抗体有序多轮孵育的循环免疫荧光成像、基于金属元素标签抗体的质谱成像、基于抗体DNA条形码的荧光成像、基于基因编码的荧光蛋白成像、基于拉曼光谱或X射线的光谱成像等单细胞蛋白质成像方法进行了总结,并对它们的成像原理作简要说明。文章重点关注了这些方法的多重性能、成像分辨率以及信号放大性能,并分析了它们在实际科研和临床工作中的应用特点,希望为新的单细胞蛋白质成像方法的开发提供参考,并为生物医学和精准医学的发展提供助力。The differential expression and subcellular localization of single-cell proteins are closely related to the physiological state and pathological mechanisms of the body.The development of single-cell protein in situ imaging methods provides powerful tools for spatial single-cell proteomics research and single-cell protein profiling.This article summarizes the single-cell protein imaging methods developed in recent years,including the circulating immunofluorescence imaging methods based on ordered multi-round antibody incubation,mass spectrometry imaging based on metal element labeled antibodies,fluorescence imaging based on DNA-barcoded antibody,gene encoded fluorescence protein imaging and spectral imaging based on Raman spectroscopy or X-ray spectroscopy,with brief explanation of the imaging principles of these methods.It focuses on the multiple performance,imaging resolution and signal amplification performance of these methods,and analyzes their application characteristics in practical scientific research and clinical work,in the hope of providing some reference for the development of more revolutionary single-cell imaging methods,and promoting the development of biomedical and precision medicine.

关 键 词：单细胞蛋白质 免疫荧光 质谱成像 荧光蛋白 光谱成像 进展 

分 类 号：R318[医药卫生—生物医学工程]