不同产蛋数岩鸽卵巢转录组的SNP分析  

SNP analysis of the ovarian transcriptome of Columba livia with different egg production numbers

作　　者：毛楠楠 孙勇胜 陈辉[1,2] 周荣艳 籍颖[1] MAO Nan-nan;SUN Yong-sheng;CHEN Hui;ZHOU Rong-yan;JI Ying(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,Hebei,China;Research Institute of Meat Pigeon Industry Technology of Hebei province,Fuping 073299,Hebei,China)

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省肉鸽产业技术研究院,河北阜平073299

出　　处：《湖北农业科学》2024年第4期185-190,共6页Hubei Agricultural Sciences

基　　金：河北省农业农村厅畜禽育种创新能力提升基金资助项目;河北省现代农业产业技术体系创新团队蛋鸡肉鸡良繁体系与品种培育岗位基金资助项目(HBCT2023210205)。

摘　　要：为探索岩鸽(Columba livia)产蛋数相关基因及其遗传标记,利用STAR对高产蛋数和低产蛋数岩鸽卵巢组织转录组数据高质量序列与参考基因组进行比对,去除重复序列后利用GATK进行单核苷酸多态性(SNP)筛选,利用VCFtools对SNP质控后进行主成分分析、进化树构建和Fst计算,利用KEGG富集分析SNP所在基因。结果显示,高产蛋组共有SNP数为298957个,低产蛋组共有SNP数为296137个,高产蛋组、低产蛋组共有SNP数为254118个,KEGG分析结果发现,SNP所在基因显著富集在孕酮调节的卵母细胞成熟、卵母细胞减数分裂和代谢等信号通路;高产蛋组和低产蛋组的102个特异SNP分布在14个注释基因上(LOC110357454、KDM5A、PIGS、BNC2、GBF1、SLAIN2、FGD4、DIAPH2、KIF3B、NFIB、EPG5、OSTF1、EMB、ST3GAL3),且富集的信号通路可能与其产蛋能力密切相关。In order to explore the genes and genetic markers related to egg production in Columba livia,STAR was used to compare high-quality transcriptome sequences of ovarian tissue transcriptome data from high and low egg production Columba livia with the ref⁃erence genome,after removing duplicate sequences,single nucleotide polymorphism(SNP)screening was performed using GATK.Af⁃ter controlling SNP quality using VCFtools,principal component analysis,evolutionary tree construction,and Fst calculation were performed.KEGG enrichment analysis was used to identify the gene where the SNP was located.The results showed that the highyielding egg group had a total of 298957 SNPs,the low yielding egg group had a total of 296137 SNPs,and the high-yielding and low-yielding egg groups had a total of 254118 SNPs.The KEGG analysis results showed that the SNP gene was significantly enriched in the progesterone regulated signaling pathways of oocyte maturation,meiosis,and metabolism;102 specific SNPs were distributed on 14 annotation genes(LOC110357454,KDM5A,PIGS,BNC2,GBF1,SLAIN2,FGD4,DIAPH2,KIF3B,NFIB,EPG5,OSTF1,EMB,and ST3GAL3)in the high and low egg production groups,and the enriched signaling pathways may be closely related to their egg production ability.

关 键 词：岩鸽(Columba livia) 卵巢转录组 SNP 产蛋数 

分 类 号：S836[农业科学—畜牧学]