基于随机森林算法筛选破裂性腹主动脉瘤的铁死亡特征基因  

Screening ferroptosis genes in ruptured abdominal aortic aneurysm based on random forest algorithm

作　　者：苗玉林 王磊[2] 田博文 丁超 李健 孙倩倩 高锋利 MIAO Yulin;WANG Lei;TIAN Bowen;DING Chao;LI Jian;SUN Qianqian;GAO Fengli(School of Clinical Medical Sciences,Ningxia Medical University,Yinchuan 750004,China;Department of Vascular Surgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Vascular Surgery,People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China)

机构地区：[1]宁夏医科大学临床医学院,宁夏银川750004 [2]宁夏医科大学总医院血管外科,宁夏银川750004 [3]宁夏回族自治区人民医院血管外科,宁夏银川750002

出　　处：《宁夏医学杂志》2024年第4期303-306,F0003,共5页Ningxia Medical Journal

摘　　要：目的探索铁死亡相关基因在稳定性腹主动脉瘤(eAAA)与破裂性腹主动脉瘤(rAAA)组织中的表达情况,筛选出rAAA的铁死亡特征基因,为进一步揭示rAAA的分子机制提供新思路。方法通过GEO数据库下载GSE98278数据集,并从FerrDb数据库下载铁死亡相关基因集(FRGs)。采用R 4.1.1软件进行数据分析,通过Limma包获得eAAA和rAAA组织中的铁死亡相关差异表达基因(FDEGs);使用clusterProfiler包和Cytoscape中的ClueGO,对FDEGs进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)功能注释分析。使用随机森林算法对FDEGs进行分类重要性排序,确定重要性评分Top 3的基因为rAAA的铁死亡特征基因,分析特征基因在eAAA和rAAA间的表达差异,并绘制诊断性ROC曲线评估其对rAAA的诊断价值。结果使用随机森林算法筛选出3个rAAA的铁死亡特征基因(SLC39A14、NDRG1和TRIM21)。相比eAAA,SLC39A14、NDRG1在rAAA组中高表达,而TRIM21在rAAA组中低表达(P<0.05)。结论SLC39A14、NDRG1和TRIM21是rAAA的铁死亡特征基因,可能为其发病机制及防治的研究提供新思路。Objective To explore the expression of genes related to ferroptosis in the tissues of elective abdominal aortic aneurysms(eAAA)and ruptured abdominal aortic aneurysm(rAAA),and to screen the characteristic genes of ferroptosis associated with rAAA,providing new insights for further revealing the molecular mechanisms of rAAA.Methods The GSE98278 dataset was downloaded from the GEO database,and the set of ferroptosis-related genes(FRGs)was obtained from the FerrDb database.Data analysis was performed by using R software version 4.1.1,and differential expression of ferroptosis-related genes(FDEGs)in eAAA and rAAA tissues were obtained through the Limma package.GO and KEGG functional annotation analyses of FDEGs were conducted by using the clusterProfiler package and Cytoscape′s ClueGO.The importance of classification for FDEGs was determined by using the random forest algorithm,identifying the top three important genes as characteristic genes of ferroptosis in rAAA.The different expressions between eAAA and rAAA were analyzed and the diagnostic ROC curves were plotted to assess their diagnostic value for rAAA.Results Three characteristic genes of ferroptosis in rAAA were identified by using the random forest algorithm(SLC39A14,NDRG1,and TRIM21).Compared to eAAA,SLC39A14 and NDRG1 were highly expressed in the rAAA group,while TRIM21 was lower expressed in the rAAA group(P<0.05).Conclusion SLC39A14,NDRG1,and TRIM21 are characteristic genes of ferroptosis in rAAA,which may provide new ideas for research on its pathogenesis and prevention.

关 键 词：破裂性腹主动脉瘤 铁死亡 随机森林 

分 类 号：R277[医药卫生—中医学]