肉芽肿性乳腺炎大鼠模型建立  

The establishment of granulomatous mastitis rat model

作　　者：马丽娜 吴晶晶[1] 陈红风[1] MA Lina;WU Jingjing;CHEN Hongfeng(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200030,China)

机构地区：[1]上海中医药大学附属龙华医院,上海市200030

出　　处：《组织工程与重建外科》2024年第2期184-189,共6页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：促进市级医院临床技能与临床创新能力三年行动计划项目(SHDC2020CR2051B)。

摘　　要：目的通过甲氧氯普胺片(胃复安)诱导血清泌乳素升高联合大鼠乳汁注射,建立肉芽肿性乳腺炎大鼠模型,并分析和评价各造模因素和指标对模型的价值,探究更符合疾病特点的动物模型。方法将16只Wistar成年雌鼠分为两组,正常对照组6只,模型组10只。对照组不予任何特殊处理;模型组连续2周予胃复安皮下注射,第1、7、14天分别予混悬液(乳汁加佐剂)乳房局部注射。每周测量一次大鼠体质量、乳腺肿块大小;在造模前、第2周及第4周,用Elisa法分别检测大鼠血清中的泌乳素水平;麻醉处死大鼠后,HE染色观察乳腺组织病理学变化;高通量液相蛋白芯片检测法测定大鼠造模前后血清中IL-1β、IL-6水平。结果造模后,模型组大鼠的皮肤、乳房肿块呈现与肉芽肿性乳腺炎患者相似的临床表现。模型组第2周泌乳素水平高于正常对照组(P<0.001),第4周仍高于正常对照组(P<0.01)。模型组肉眼可见部分肿块内粉刺样物质,镜下病变区可见多灶散在分布上皮样细胞构成肉芽肿性结节,有多种炎细胞浸润,以淋巴细胞为主,另见泡沫样组织细胞、浆细胞、多核巨细胞、巨噬细胞等。模型组大鼠IL-1β、IL-6水平均高于造模前(P<0.01)。结论采用胃复安诱导血清泌乳素升高联合大鼠乳汁注射的方法,在造模4周后能成功建立肉芽肿性乳腺炎模型。Objective To establish a rat model of granulomatous mastitis through the combination of gastrin-induced elevation of serum lactogen and rat milk injection,and to analyze and evaluate the value of each modeling factor and index on the model,and to explore an animal model that better fits the characteristics of the disease.Methods Sixteen adult female Wistar rats were divided into two groups:6 rats in the normal control group and 10 rats in the model group.The normal control group was not given any special treatment.Rats in the model group were injected subcutaneously with gastrin for 2 weeks,and the rats were injected locally with a suspension(milk plus adjuvant)on the 1st,7th and 14th days respectively.The changes in body weight and mammary mass size were measured once a week.Serum prolactin levels were detected by ELISA before,at the 2nd and 4th week of modeling.After the rats were executed,the histopathological changes of mammary glands were observed by HE staining.The serum levels of IL-1βand IL-6 were measured by high-throughput liquid-phase protein microarray assay before and after modeling.Results After modeling,the skin and breast lumps of the model rats showed similar clinical manifestations as those of patients with granulomatous mastitis.In terms of serum prolactin level,the PRL level of the model group was higher than that of the normal control group at week 2(P<0.001);The PRL level of the model group was still higher than that of the normal control group at week 4(P<0.01).In terms of pathomorphology,some of the masses were visible as acne-like material to the naked eye,and microscopically the lesion areas were seen as multifocal scattered distribution of epithelioid cells constituting granulomatous nodules with a variety of inflammatory cell infiltrates,mainly lymphocytes,and foamy histiocytes,plasma cells,multinucleated giant cells and macrophages.The levels of serum interleukin 1β(IL-1β)and interleukin 6(IL-6)after modeling were higher than before modeling(P<0.01).Conclusion Gastrin-induced eleva

关 键 词：肉芽肿性乳腺炎 泌乳素 分泌物 WISTAR大鼠 

分 类 号：R655.8[医药卫生—外科学]