2个佛手瓜品种核型分析及基因组大小测定  

Karyotyping Analysis and Genome Size Determination of Two Sechium edule Varieties

作　　者：王维泽 刘炼 赵宁[1] 瞿飞 黎瑞君[2] 文林宏[1] 张朝君[1] 陈之林[1] 李裕荣 WANG Weize;LIU Lian;ZHAO Ning;QU Fei;LI Ruijun;WEN Linhong;ZHANG Chaojun;CHEN Zhilin;LI Yurong(Guizhou Institute of Horticulture,Guiyang 550006,China;Guizhou Agricultural Science and Technology Information Institute,Guiyang 550006,China)

机构地区：[1]贵州省园艺研究所,贵阳550006 [2]贵州省农业科技信息研究所,贵阳550006

出　　处：《种子》2024年第3期123-128,136,共7页Seed

基　　金：贵州省科技计划项目“佛手瓜新品系选育及优良种苗繁育技术研究”(黔科合支撑[2021]一般210);贵州省科技计划项目“贵州省高层次创新型人才培养计划——“百”层次人才”(黔科合平台人才[2016]5671号);贵州省科技计划项目“园艺植物基因库创新平台建设及利用”(黔科合服企[2022]005-H-2);贵州省科技计划项目“贵州石山区佛手瓜套种春白菜种植模式研究与示范”(黔科合支撑[2021]一般211)。

摘　　要：本试验以贵州省惠水县主要品种绿皮佛手瓜、白皮佛手瓜为材料,通过去壁低渗法染色体制片、流式细胞术分别对其核型、基因组大小,染色体数量、倍性等特征进行研究。结果表明,贵州惠水白皮佛手瓜、绿皮佛手瓜均为2倍体,染色体数目均为28条,白皮佛手瓜、绿皮佛手瓜的核型公式均为2n=2x=28m,白皮佛手瓜核型类型为1B型,绿皮佛手瓜为1C型,绿皮佛手瓜的不对称系数为52.38%,白皮佛手瓜的不对称系数为52.07%,通过流式细胞术测定,白皮佛手瓜、绿皮佛手瓜基因组大小分别为(0.73±0.02)Gb、(0.70±0.02)Gb。In this study,the karyotype,genome size,chromosome number and ploidy of the main varieties of green and white Sechium edule in Huishui County,Guizhou Province were studied by chromosome dissection and flow cytometry.The results showed that both white-skinned and green-skinned Sechium edule were diploid and had 28 chromosomes.The karyotype formulas of white-skinned Sechium edule and green-skinned Sechium edule were 2n=2x=28m,the karyotype of white-skinned Sechium edule was type 1B,and that of green-skinned Sechium edule was type 1C.The asymmetry coefficient of green-skinned Sechium edule was 52.38%,and the asymmetry coefficient of white-skinned Sechium edule was 52.07%,by flow cytometry,the genome size of white-skinned and green-skinned Sechium edule was(0.73±0.02)Gb and(0.70±0.02)Gb,respectively.

关 键 词：白皮佛手瓜 绿皮佛手瓜 染色体 流式细胞术 

分 类 号：S642.9[农业科学—蔬菜学]