粉防己组织培养体系的建立和细胞悬浮培养初探  

Establishment of Plant Tissue Culture and Cell Suspension Culture System of Stephania tetrandra S.Moore

作　　者：李萍 陈荣秀 李文兰 LI Ping;CHEN Rongxiu;LI Wenlan(College of Life Science and Technology,Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Nanning 530004,China)

机构地区：[1]广西大学生命科学与技术学院/亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《种子》2024年第3期137-142,共6页Seed

基　　金：国家自然科学基金(31560247)。

摘　　要：为促进粉防己的资源保护和利用,以茎段和愈伤组织为材料建立植物组织培养体系,并利用愈伤组织细胞进行悬浮培养。结果表明,以茎段为外植体直接分化不定芽的最佳培养基为MS+6-BA 1.5 mg/L+NAA 0.2 mg/L,60 d不定芽诱导率为100%,单个外植体分化不定芽数16个,90 d时分化数可达28个,该配方同时适用于诱导愈伤组织分化不定芽;不定芽生根以培养基MS+IAA 0.2 mg/L为佳,30 d的生根率达92.5%,平均主根长7.78 cm,炼苗移栽15 d的成活率达88.00%;利用愈伤组织细胞进行悬浮培养,可在较短的培养周期获得可观的生物积累量,干培养物提取液生物碱定性检测为阳性。In order to promote the protection and utilization of the resources of Stephania tetrandra S.Moore,a plant tissue culture system was established using stem segments and callus tissue as materials,and suspension culture was carried out using callus tissue cells.Result showed that the optimal culture medium for direct differentiation of adventitious buds from stem segments was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L.The induction rate of adventitious buds was 100%after 60 days,with 16 adventitious buds differentiated from a single explant and up to 28 at 90 days.This formula was also suitable for inducing callus tissue differentiation of adventitious buds,MS+IAA 0.2 mg/L was the best method for adventitious bud rooting,with a rooting rate of 92.5%after 30 days,an average main root length of 7.78 cm,and a survival rate of 88.00%after 15 days of seedling cultivation and transplantation.Suspension culture using callus cells could achieve significant bioaccumulation in a shorter culture cycle,and the qualitative detection of alkaloids in dry culture extract was positive.

关 键 词：粉防己 组织培养快繁 细胞悬浮培养 次生代谢产物 

分 类 号：S567.239[农业科学—中草药栽培]