不同干燥方式的九制何首乌对D-半乳糖致衰老小鼠的抗衰老作用  

Anti-Aging Effects of Nine-Time Processed Polygonum Multiflorum Thunb with Different Drying Methods on D-Galactose-Induced Aging Mice

作　　者：任娟 姜誉弘 刘史佳 俞凡 庞会明 REN Juan;JIANG Yuhong;LIU Shijia;YU Fan;PANG Huiming(Department of Pharmacy,Affiliated Hospital of Nanjing University of Chinese Medicine&Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院/江苏省中医院药学部,南京210029

出　　处：《药学与临床研究》2024年第2期109-114,共6页Pharmaceutical and Clinical Research

基　　金：国家中医临床研究基地(江苏省中医院)开放课题项目(JD2019SZ14);江苏省中医药科技发展专项(2020ZX11)。

摘　　要：目的:探究不同干燥方式的九制何首乌对D-半乳糖诱导衰老小鼠的影响。方法:将50只ICR小鼠随机分成空白组、模型组、阳性对照组、九蒸九晒制首乌组、九蒸九烘制首乌组,每日腹腔注射D-半乳糖(0.5 g·kg^(-1))构建小鼠衰老模型,造模同时给药,持续12周。应用Morris水迷宫实验方法测定小鼠的空间记忆和学习水平,计算各组小鼠胸腺、脾脏指数,检测小鼠血清及脑、肝、肾匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平,检测小鼠肝肾功能水平。结果:与空白对照组相比,模型组空间记忆和学习能力明显降低,体质量、脏器指数明显下降,血清以及全脑、肝、肾匀浆中SOD、GSH-Px水平明显下降,而MDA水平显著上升,血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、肌酐、尿素水平显著性上升;药物干预组与模型组相比,空间记忆和记忆水平明显改善,体质量、脏器系数显著增加,改善D-半乳糖诱导的氧化应激,提高SOD、GSH-Px水平,抑制MDA含量的升高,肝肾功能趋于正常。两种干燥方式的给药组小鼠的指标相当,无明显差异。结论:不同干燥方法的九制何首乌的水煎液对D-半乳糖致衰老小鼠具有抗衰老作用,且两种方法的抗衰老作用无显著差异。Objective:To explore the effects of nine-time processed Polygonum Multiflorum Thunb with different drying methods on D-galactose-induced aging mice.Methods:Fifty ICR mice were randomly divided into blank control group,model group,positive control group,nine-time repeated steaming and sun-drying group,and nine-time repeated steaming and stove-drying group.Aging model was established through intraperitoneal injection of D-galactose(0.5 g·kg^(-1))every day.Mice in each group were intragastrically administered with drugs respectively for 12 weeks.For mice in each group,Morris water maze experiments were used to test their spatial memory and learning ability;the indexes of thymus and spleen were calculated;the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)and malondialdehyde(MDA)in serum and homogenates of whole brain,liver and kidney were determined;liver and kidney functions were detected.Results:Compared with the normal control group,the model group showed significant decrease in spatial memory,learning ability,body mass and organ index;the levels of SOD and GSH-Px in serum and homogenates of whole brain,liver and kidney decreased significantly,while the MDA levels increased significantly;the serum levels of ALT,ALP,AST,CREA and UA increased markedly.Compared with the model group,the drug intervention groups significantly improved spatial memory and learning ability,significantly increased body mass and organ index,improved oxidative stress induced by D-galactose,increased SOD and GSH-Px levels,inhibited the increase of MDA contents,and normalized liver and kidney functions.The indexes of mice between the groups of two drying methods were similar with no significant difference.Conclusion:The water decoction of nine-time processed Polygonum Multiflorum Thunb with different drying methods has anti-aging effects on D-galactose-induced aging mice,and there is no significant difference in anti-aging effects between the two drying methods.

关 键 词：九制何首乌 晒干 烘干 抗衰老 D-半乳糖 

分 类 号：R943.1[医药卫生—药剂学]