太子参改善斑马鱼和APP/PS 1小鼠学习记忆  

Improving effect of Pseudostellaria heterophylla on learning and memory of zebrafish and APP/PS 1 mice

作　　者：丰心月 王奕霏 邓嘉航 何传统 蒋嘉慧 杨志友 FENG Xinyue;WANG Yifei;DENG Jiahang;HE Chuantong;JIANG Jiahui;YANG Zhiyou(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Key Laboratory of Aquatic Product Processing and Safety,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东海洋大学水产品加工与安全重点实验室,广东湛江524088

出　　处：《食品与发酵工业》2024年第8期55-61,共7页Food and Fermentation Industries

基　　金：广东省自然科学基金面上项目(2022A1515011419);广东省教育厅重点领域专项(2022ZDZX2029);广东省大学生创新创业训练计划项目(S202110566015)。

摘　　要：太子参是一种可用于保健食品的传统中药,具有抗疲劳和调节免疫等作用,但神经保护和改善记忆作用报道较少。为探究太子参改善记忆和认知障碍的有效成分及作用机制,利用Aβ1-42脑室显微注射斑马鱼模型初步探究太子参醇提物对记忆的改善作用;CCK8试剂盒测定太子参水提物、醇提物、多糖、皂苷、环肽对Aβ25-35诱导皮层神经元存活率的影响;荧光定量PCR测定太子参环肽B(heterophyllin B,HB)对神经元中凋亡相关基因的表达,免疫细胞化学染色探究HB对神经元的保护作用;利用APP/PS 1转基因小鼠探究HB对痴呆样行为和记忆的改善作用。太子参醇提物给药后,斑马鱼新臂和奖励臂探寻的潜伏期显著降低,在新臂和奖励臂游动时间和总路程增加。HB显著增加神经元存活率及β3-tubulin、MAP2阳性突起的表达,并改善APP/PS 1转基因小鼠记忆障碍。综上,太子参的神经保护作用可能通过HB减少突起萎缩,从而改善认知功能障碍和记忆缺陷。Pseudostellaria heterophylla is a kind of traditional medicine as well as healthy food,which exerts anti-fatigue and immune-regulative effects,however,the neuroprotective and memory ameliorative effects are rarely reported.To explore the effective components and their mechanism in AD,the highly efficient zebrafish AD model was performed to evaluate the neuroprotective effects of Pseudostellaria heterophylla ethanol extract,cell counting kit-8(CCK8)was used to determine the effects of aqueous extracts,ethanol extracts,polysaccharides,saponins,and cyclopeptides of Pseudostellaria heterophylla on cortical neuronal survival.Fluorescent quantitative PCR was used to detect the expression of apoptosis-related genes in neurons after intervention with heterophyllin B(HB),and immunocytochemical staining was used to investigate the neuroprotective effects.Behavioral tests were conducted to validate the memory ameliorative effects in APP/PS 1 transgenic mice.Compared with Aβ-cerebroventricular microinjection zebrafish model(CVMI),Pseudostellaria heterophylla ethanol extract treatment reduced the latency to the new arm and reward arm,increased the swimming time and total distance in the new arm and reward arm,and significantly increased the survival rate in Aβ-induced primary cultured neurons.HB significantly improved memory function in APP/PS 1 transgenic mice,as well as enhanced neuronal survival and elongated neurites in cultured neurons.Collectively,HB was identified as one of the effective constituents of Pseudostellaria heterophylla,which exerts neuroprotective effects,thus leading to neural network reconstruction and memory improvement.

关 键 词：阿尔兹海默症 Β-淀粉样蛋白 太子参环肽B 神经突起 记忆障碍 

分 类 号：R285.5[医药卫生—中药学]