基于硼掺杂氮化碳材料构建ECL生物传感器用于MicroRNA的灵敏检测  

Construction of ECL biosensor based on B-doped graphitic carbon nitride for sensitive detection of microRNA

作　　者：徐苑棋 袁若[1] Xu Yuan-qi;Yuan Ruo(College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《化学传感器》2023年第4期44-53,共10页Chemical Sensors

基　　金：国家级大学生创新创业训练项目(202310635097、202310635042)。

摘　　要：研究中,用所获得的硼掺杂氮化碳(B-C_(3)N_(4))作为发射体,构建了一种用于超灵敏检测疾病标志物microRNA-222(miRNA-222)的ECL生物传感器。首先,将制得的B-C_(3)N_(4)材料修饰在玻碳电极(GCE)表面成膜,再在电极表面修饰上金纳米粒子(Au NPs)来固载DNA以实现后续传感器的构建。所设计的ECL生物传感器对miRNA-222具有良好的ECL信号响应,实现了对miRNA-222的超灵敏检测,其线性范围为1 fmol/L~1 nmol/L,并成功应用于癌症细胞裂解液中miRNA-222的检测。该项工作丰富了非金属纳米材料在ECL领域的应用,并为在临床和生物化学中的microRNA超灵敏检测提供了新的途径。In this study,the obtained B-doped C_(3)N_(4)(B-C_(3)N_(4))as emitter was used for the construction of ECL biosensor to achieve the ultra-sensitive detection of microRNA-222(miRNA-222).Firstly,the prepared B-C_(3)N_(4) was modified on the surface of the glassy carbon electrode to form a film,and then gold nanoparticles(Au NPs)were modified on the electro de surface to immobilize DNA for subsequent biosensor construction.The designed ECL biosensor exhibited good ECL signal to achieve ultra-sensitive detection of miRNA-222 with a linear range of 1 fmol/L~1 nmol/L,and it was successfully applied to miRNA-222 in cancer cell lysates.This work enriched the application of non-metallic nanomaterials in the field of ECL and provided a new approach for ultra-sensitive detection of miRNA in clinical and biochemical fields.

关 键 词：电化学发光传感器 硼掺杂氮化碳 MICRORNA 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]